Gene Ontology annotation through association of InterPro records with GO terms
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InterPro2GO maps generic cytochrome P450 signatures to monooxygenase, heme binding, iron ion binding and paired-donor oxidoreductase terms; all correct but non-core relative to the specific homomethionine N-monooxygenase activity.
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Cytochrome p450 CYP79F1 from arabidopsis catalyzes the conversion of dihomomethionine and trihomomethionine to the corresponding aldoximes in the biosynthesis of aliphatic glucosinolates.
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Recombinant CYP79F1 (E. coli) converts dihomomethionine and trihomomethionine to 5-methylthiopentanaldoxime and 6-methylthiohexanaldoxime; cosuppression lines have reduced aliphatic glucosinolates, accumulate the substrates, and are bushy.
"Using recombinant CYP79F1 functionally expressed in Escherichia coli, we show that both dihomomethionine and trihomomethionine are metabolized by CYP79F1 resulting in the formation of 5-methylthiopentanaldoxime and 6-methylthiohexanaldoxime, respectively."
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CYP79F1 cosuppression causes loss of apical dominance and multiple axillary shoots, an indirect developmental consequence of the metabolic block.
"The transgenic plants have a morphological phenotype showing loss of apical dominance and formation of multiple axillary shoots."
Bus, a bushy Arabidopsis CYP79F1 knockout mutant with abolished synthesis of short-chain aliphatic glucosinolates.
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bus1-1f null mutants completely lack short-chain methionine-derived glucosinolates and overexpression increases them; N-terminal GFP fusions of CYP79F1 and CYP79F2 localize to the ER.
"Short-chain glucosinolates derived from methionine were completely lacking in the null mutant and showed increased levels in the overexpressing plant"
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The bushy phenotype is attributed to altered methionine-derived intermediates or to increased indole glucosinolates/IAA, i.e. indirect effects of the metabolic block.
"The bus phenotype may result from either an altered content of methionine-derived glucosinolates and their biosynthetic intermediates or from an increase of indolyl glucosinolates and IAA concentrations."
CYP79F1 and CYP79F2 have distinct functions in the biosynthesis of aliphatic glucosinolates in Arabidopsis.
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CYP79F1 metabolizes mono- to hexahomomethionine whereas CYP79F2 only metabolizes penta- and hexahomomethionine; cyp79f1 knockout lacks short-chain aliphatic glucosinolates.
"we show that CYP79F1 metabolizes mono- to hexahomomethionine, resulting in both short- and long-chain aliphatic glucosinolates."
Functional analysis of the tandem-duplicated P450 genes SPS/BUS/CYP79F1 and CYP79F2 in glucosinolate biosynthesis and plant development by Ds transposition-generated double mutants.
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cyp79f1 cyp79f2 double knockouts completely lack aliphatic glucosinolates; sps/cyp79f1 shoot overproliferation correlates with elevated cytokinin, an indirect hormonal consequence.
"Our analysis shows that aliphatic glucosinolate biosynthesis is completely abolished in the double-knockout plants, providing genetic proof for the proposed biochemical functions of these genes."
Modulation of CYP79 genes and glucosinolate profiles in Arabidopsis by defense signaling pathways.
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CYP79F1 and CYP79F2 are induced by methyl jasmonate.
"CYP79F1 and CYP79F2 , which are involved in biosynthesis of aliphatic glucosinolates, were induced by MeJA."
Water stress and aphid feeding differentially influence metabolite composition in Arabidopsis thaliana (L.).
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Aphid infestation decreased transcripts of MAM1, CYP79F1, CYP83A1 and UGT74C1.
"transcript levels of biosynthetic genes MAM1, CYP79F1, CYP83A1, and UGT74C1 decreased after infestation with either aphid species"
Global Analysis of Membrane-associated Protein Oligomerization Using Protein Correlation Profiling.
Impact of Lepidopteran Oral Secretions on the Transcriptome of Arabidopsis thaliana.
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Caterpillar oral secretions suppress aliphatic glucosinolate biosynthetic gene expression, including CYP79F1 and CYP79F2 (qPCR).
"Strikingly, OS of both caterpillars suppressed the basal expression of MYC4, MYB28, and MYB29 and most genes responsible for aliphatic GS biosynthesis (Figure 6A), three of which were further validated by qPCR (Figure 6B)."
UniProtKB entry Q949U1 (CYP79F1, Dihomomethionine N-hydroxylase)