Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic Gene Ontology annotation based on Rhea mapping
Combined Automated Annotation using Multiple IEA Methods
Implications of the structure of human uridine phosphorylase 1 on the development of novel inhibitors for improving the therapeutic window of fluoropyrimidine chemotherapy.
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High-resolution crystal structures confirm that the active human uridine phosphorylase 1 is a homodimer, unlike the hexameric microbial UPPs, and define the active-site interactions used for inhibitor (BAU) design.
"is dimeric in contrast to the hexameric assembly present in microbial UPPs"
Active site conformational dynamics in human uridine phosphorylase 1.
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Structure of hUPP1 bound to 5-fluorouracil confirms the strictly dimeric eukaryotic assembly and reveals inter-domain hinge motion coupling the two active sites during catalysis.
"strictly dimeric complexes in eukaryotic organisms"
Cloning and expression of human uridine phosphorylase.
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Cloned human UPP1 (from colorectal tumor line HCT116); recombinant enzyme showed specific uridine phosphorylase activity inhibited by a competitive uridine-analogue inhibitor, and expression was induced by vitamin D3 and inflammatory cytokines.
"specific enzyme activity with uridine as the substrate"
uracil + (deoxy)ribose 1-phosphate <=> (deoxy)uridine + orthophosphate [UPP]
(deoxy)uridine + orthophosphate <=> uracil + (deoxy)ribose 1-phosphate (UPP)
Targeted deletion of both thymidine phosphorylase and uridine phosphorylase and consequent disorders in mice.
Purification, cloning, and expression of murine uridine phosphorylase.
Abnormalities in uridine homeostatic regulation and pyrimidine nucleotide metabolism as a consequence of the deletion of the uridine phosphorylase gene.
Uridine phosphorylase (-/-) murine embryonic stem cells clarify the key role of this enzyme in the regulation of the pyrimidine salvage pathway and in the activation of fluoropyrimidines.
Identification of a liver-specific uridine phosphorylase that is regulated by multiple lipid-sensing nuclear receptors.
SPECIFICITY OF MOUSE URIDINE PHOSPHORYLASE. CHROMATOGRAPHY, PURIFICATION, AND PROPERTIES.
Uridine protects cortical neurons from glucose deprivation-induced death: possible role of uridine phosphorylase.