Use of the ND evidence code for Gene Ontology (GO) terms
Annotation inferences using phylogenetic trees
The C. elegans homolog of the murine cystic kidney disease gene Tg737 functions in a ciliogenic pathway and is disrupted in osm-5 mutant worms.
-
OSM-5 encodes a TPR-repeat containing protein homologous to murine polaris
"osm-5 encodes a tetratricopeptide repeat (TPR)-containing protein that is the homolog of murine polaris (Tg737)"
-
OSM-5 is expressed in ciliated sensory neurons under DAF-19 regulation
"osm-5 is expressed in ciliated sensory neurons in C. elegans and its expression is regulated by DAF-19, an RFX-type transcription factor"
-
OSM-5::GFP localizes to cilium base and within cilium axoneme
"the OSM-5 protein was found to concentrate at the cilium base and within the cilium axoneme as shown by an OSM-5::GFP translational fusion and immunofluorescence"
-
OSM-5::GFP shows fluorescent particle migration within cilia (IFT movement)
"time-lapse imaging of OSM-5::GFP fusion protein shows fluorescent particle migration within the cilia"
-
osm-5 mutants have ciliary defects rescued by wild-type gene
"mutations in the Caenorhabditis elegans gene Y41g9a.1 are responsible for the ciliary defects in osm-5 mutant worms. This was confirmed by transgenic rescue of osm-5(p813) mutants using the wild-type Y41g9a.1 gene"
Basal body dysfunction is a likely cause of pleiotropic Bardet-Biedl syndrome.
-
BBS proteins localize to basal bodies and function in ciliogenesis
"BBS8 localizes specifically to ciliated structures, such as the connecting cilium of the retina and columnar epithelial cells in the lung. In cells, BBS8 localizes to centrosomes and basal bodies and interacts with PCM1, a protein probably involved in ciliogenesis"
-
C. elegans BBS homologs are expressed exclusively in ciliated neurons
"all available Caenorhabditis elegans BBS homologues are expressed exclusively in ciliated neurons"
Sensory ciliogenesis in Caenorhabditis elegans: assignment of IFT components into distinct modules based on transport and phenotypic profiles.
-
OSM-5 assigned to IFT-B module based on transport profiles
"the C. elegans IFT machinery has a modular design, consisting of modules IFT-subcomplex A, IFT-subcomplex B, and a BBS protein complex"
-
IFT machinery has modular design with IFT-A, IFT-B, and BBS modules
"the C. elegans IFT machinery has a modular design, consisting of modules IFT-subcomplex A, IFT-subcomplex B, and a BBS protein complex, in addition to motor and cargo modules"
-
osm-5 mutants analyzed for cilium and IFT phenotypes
"We also analyzed the distribution and transport of fluorescent IFT particles in multiple known ciliary mutants and 49 new ciliary mutants"
Genetic analysis of chemosensory control of dauer formation in Caenorhabditis elegans.
-
osm-5 is one of nine genes causing structurally defective chemosensory cilia
"Dauer-defective mutations in nine genes cause structurally defective chemosensory cilia, thereby blocking chemosensation"
-
Cilium-structure gene mutations block chemosensation and dauer formation
"Mutations in all nine of these genes appear to fall at a single step in the epistasis pathway"
-
Genetic epistasis places osm-5 at a single step with other ciliary genes
"Mutations in all nine of these genes appear to fall at a single step in the epistasis pathway"
Mutation of the MAP kinase DYF-5 affects docking and undocking of kinesin-2 motors and reduces their speed in the cilia of Caenorhabditis elegans.
-
IFT proteins including OSM-5 visualized in C. elegans cilia
"In the cilia of the nematode Caenorhabditis elegans, anterograde intraflagellar transport (IFT) is mediated by two kinesin-2 complexes"
-
Anterograde IFT mediated by kinesin-II and OSM-3 kinesin
"In the cilia of the nematode Caenorhabditis elegans, anterograde intraflagellar transport (IFT) is mediated by two kinesin-2 complexes"
The BBSome controls IFT assembly and turnaround in cilia.
-
IFT-B components assemble at ciliary base with BBSome regulation
"the BBSome (refs 3, 4), a group of conserved proteins affected in human Bardet-Biedl syndrome(5) (BBS), assembles IFT complexes at the ciliary base"
-
IFT-A and IFT-B associate for anterograde transport
"the BBSome is still functional at the ciliary base supported by the observation that IFT-A and IFT-B associate in anterograde transport"
-
BBSome required for IFT particle assembly and recycling
"we conclude that the BBSome is required for assembling IFT particles at both ciliary base and tip"
Mutant sensory cilia in the nematode Caenorhabditis elegans.
-
osm-5(p813) mutants have normal transition zones but severely shortened axonemes
"The cilia in che-13 (e1805), osm-1 (p808), osm-5 (p813), and osm-6 (p811) mutants have normal transition zones and severely shortened axonemes"
-
Doublet-microtubules assemble ectopically proximal to cilia in osm-5 mutants
"Doublet-microtubules, attached to the membrane by Y links, assemble ectopically proximal to the cilia in these mutants"
-
osm-5 affects many or all sensory cilia in the head
"Ten genes affect many or all of the sensory cilia in the head"
Whole-Organism Developmental Expression Profiling Identifies RAB-28 as a Novel Ciliary GTPase Associated with the BBSome and Intraflagellar Transport.
-
osm-5 used as ciliary mutant control in sensory behavioral assays
"osm-5(p813) worms used as a negative control"
-
Ciliated neurons require IFT for proper sensory function
"Cilium formation, maintenance and function depend on intracellular transport systems such as intraflagellar transport (IFT)"
Dynein-Driven Retrograde Intraflagellar Transport Is Triphasic in C. elegans Sensory Cilia.
-
IFT-B complex components analyzed for ciliary localization
"Disruption of the dynein-2 tail domain, light intermediate chain, or intraflagellar transport (IFT)-B complex abolishes dynein-2's ciliary localization"
-
Anterograde and retrograde IFT characterized in sensory cilia
"Cytoplasmic dynein-2 powers retrograde intraflagellar transport that is essential for cilium formation and maintenance"
Deep research report on osm-5