GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

InterPro mappings supplied broad transcription, catabolism, and oxidoreductase annotations reviewed here.

GO_REF:0000108

Automatic assignment of GO terms using logical inference, based on on inter-ontology links

Logical inference supplied negative regulation from the repressor molecular function.

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

ARBA supplied DNA-binding, repressor, membrane-side, and generic binding predictions reviewed here.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

Combined automated mappings supplied both catalytic activities, membrane association, and the erroneous biosynthesis process.

PMID:10613867

Proline catabolism by Pseudomonas putida: cloning, characterization, and expression of the put genes in the presence of root exudates.

Direct P. putida genetics links PutA to growth on proline; sequence homology assigns the PRODH and GSALDH regions within the fused protein.

"both enzymatic steps for proline conversion to glutamic acid are catalyzed by a single polypeptide"

PMID:11097893

Control of expression of divergent Pseudomonas putida put promoters for proline catabolism.

Direct promoter studies establish PutA-dependent repression independent of catalytic activity.

"This regulatory activity is independent of the catabolic activity of PutA"

PMID:18767154

Solution structure of the Pseudomonas putida protein PpPutA45 and its DNA complex.

The P. putida PutA RHH domain binds a defined put control DNA oligomer.

"PpPutA45 was shown to bind a 14 base-pair DNA oligomer"

PMID:28712849

Structure, function, and mechanism of proline utilization A (PutA).

The review supports the conserved two-step chemistry and redox-dependent localization switch of PutA proteins.

"FAD-dependent proline dehydrogenase (PRODH) and NAD+-dependent glutamate semialdehyde dehydrogenase (GSALDH) convert proline to glutamate in two sequential oxidative steps."

file:PSEPK/putA/putA-uniprot.txt

UniProtKB entry Q88D80 for Pseudomonas putida KT2440 PutA

The exact record assigns both catalytic reactions, FAD, the PutA RHH domain, and the proline-degradation pathway.

file:PSEPK/putA/putA-goa.tsv

QuickGO GOA annotations for putA

file:PSEPK/putA/putA-deep-research-openscientist.md

OpenScientist deep research report on putA (Q88D80)

The generated report retrieved direct P. putida studies and family-level PutA mechanistic literature used for source discovery.