Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Hedgehog movement is regulated through tout velu-dependent synthesis of a heparan sulfate proteoglycan.
Structural analysis of glycosaminoglycans in Drosophila and Caenorhabditis elegans and demonstration that tout-velu, a Drosophila gene related to EXT tumor suppressors, affects heparan sulfate in vivo.
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ttv mutant larvae have reduced heparan sulfate but not chondroitin sulfate.
"Analysis of total glycosaminoglycans from tout-velu mutant larvae show marked reductions in heparan sulfate but not chondroitin sulfate"
Structural analysis of glycosaminoglycans in animals bearing mutations in sugarless, sulfateless, and tout-velu. Drosophila homologues of vertebrate genes encoding glycosaminoglycan biosynthetic enzymes.
Demonstration of a novel gene DEXT3 of Drosophila melanogaster as the essential N-acetylglucosamine transferase in the heparan sulfate biosynthesis: chain initiation and elongation.
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Botv/DEXT3 (EXTL3 ortholog) has GlcNAcT-I and GlcNAcT-II activity; it, not Ttv, initiates HS chains.
"The results suggest that DEXT3 is the ortholog of human EXTL3"
Three Drosophila EXT genes shape morphogen gradients through synthesis of heparan sulfate proteoglycans.
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ttv, sotv and botv are each non-redundantly required for HSPG biosynthesis and for Hh, Dpp and Wg morphogen distribution.
"Mutation in any of these genes impaired biosynthesis of HSPGs in vivo"
Distinct and collaborative roles of Drosophila EXT family proteins in morphogen signalling and gradient formation.
Abrogation of heparan sulfate synthesis in Drosophila disrupts the Wingless, Hedgehog and Decapentaplegic signaling pathways.
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HS is dramatically reduced in ttv and sotv mutants; EXT1 and EXT2 are the human orthologs of ttv and sotv.
"HS levels are dramatically reduced in the absence of Sotv or its partner co-polymerase Tout velu (Ttv)"
Heparan sulfate polymerization in Drosophila.
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Ttv has GlcNAcT-II and GlcAT-II activities, augmented by Sotv; Ttv-Sotv lacks GlcNAcT-I activity.
"In contrast to human, TTV-SOTV exhibited no GlcNAcT-I activity"
toutvelu, a regulator of heparan sulfate proteoglycan biosynthesis, controls guidance cues for germ-cell migration.
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Reported germ cell migration defects in ttv loss- and gain-of-function embryos, attributed to somatic Hh distribution.
"it seems likely that ttv activity is required in the soma, not the germline"
Hedgehog does not guide migrating Drosophila germ cells.
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ttv null maternal-only or zygotic-only mutants have normal germ cell migration; ttv overexpression did not cause migration defects.
"Taken together our results suggest that ttv loss of function has no effect on germ cell migration."
Cell type-specific requirements for heparan sulfate biosynthesis at the Drosophila neuromuscular junction: effects on synapse function, membrane trafficking, and mitochondrial localization.
Tout-velu is a Drosophila homologue of the putative tumour suppressor EXT-1 and is needed for Hh diffusion.
Drosophila glypicans control the cell-to-cell movement of Hedgehog by a dynamin-independent process.
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The glypicans Dally and Dally-like are substrates of Ttv in vivo.
"two Drosophila glypican members of the heparan sulphate proteoglycan (HSPG) family, are the substrates of Ttv"
The Drosophila GOLPH3 homolog regulates the biosynthesis of heparan sulfate proteoglycans by modulating the retrograde trafficking of exostosins.
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Rotini/GOLPH3 controls intra-Golgi retrograde trafficking and stability of EXTs.
"Here, we show that Rotini (Rti), the Drosophila GOLPH3, regulates the retrograde trafficking of EXTs."
Cholesterol modification of hedgehog is required for trafficking and movement, revealing an asymmetric cellular response to hedgehog.
UniProt entry Q9V730 (EXT1_DROME)