Gene Ontology annotation through association of InterPro records with GO terms
Combined Automated Annotation using Multiple IEA Methods
Self-poisoning of Mycobacterium tuberculosis by targeting GlgE in an alpha-glucan pathway.
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Experimental work in Mycobacterium established the conserved four-step TreS-Mak-GlgE-GlgB route and identifies maltose 1-phosphate as the GlgE donor; it supports the pathway architecture but is not a direct assay of Pseudomonas TreSB.
"We describe a new pathway from trehalose to alpha-glucan in Mycobacterium tuberculosis comprising four enzymatic steps mediated by TreS, Pep2, GlgE (which has been identified as a maltosyltransferase that uses maltose 1-phosphate) and GlgB."
OpenScientist functional-annotation report for treSB
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The report corroborates the fused TreS and maltokinase architecture, exact two-reaction assignment, and position upstream of GlgE while distinguishing direct target evidence from ortholog-derived mechanism.
UniProtKB entry for treSB (Q88FN0)
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UniProt assigns Q88FN0 to PANTHER subfamily PTHR10357:SF219 (MALTOSE ALPHA-D-GLUCOSYLTRANSFERASE).
"PTHR10357:SF219; MALTOSE ALPHA-D-GLUCOSYLTRANSFERASE"
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The unreviewed record assigns two reactions to one 1106-aa protein: EC 5.4.99.16/RHEA:15145 for maltose-trehalose interconversion and EC 2.7.1.175/RHEA:31915 for ATP-dependent maltose phosphorylation.
PANTHER PAINT annotations for PTHR10357
PANTHER PTHR10357 family entries
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The experimentally characterized PAINT seed P9WQ19 (Mycobacterium TreS) is a member of PTHR10357:SF219, the same subfamily that UniProt assigns to Q88FN0.
"PTHR10357:SF219,MALTOSE ALPHA-D-GLUCOSYLTRANSFERASE"
UniProtKB entry for adjacent glgE (Q88FM9)