The experimental construct and feedback synthesis are now in the appended biological notes. Original Methods/Results and Figure4 locators remain in the independent evidence.
Human E6S-reference and E111V proteins were compared in vitro. Structural simulations and aggregation measurements do not establish additional normal functions.
The clinical variants are human, but the functional study used bovine GCAP1 constructs and washed bovine outer-segment membranes.
Normal cache: abstract only. Original Methods94โ148 and human Results221โ227 were independently read. The antigen was bovine GCAP1; inner-retinal GCAP2 staining is distinct.
PubMed record ยท Original article, Methods and human Results.
Each pair has three curated Y2H-method records from one HuRI study. These support assay association, not three independent studies or verified endogenous complexes. Uncertain native encounter does not negate the supported physical-association claim. Partner UniProt records are secondary summaries; their cited papers were not newly read. No more-specific GCAP1 molecular function follows from these associations.
Indexed original Methods and wild-type controls were read. Bovine GCAP1 was produced in bacteria; human RetGC1 was expressed in HEK293 cells for biochemical assays. Mutant structural differences are not assigned to wild-type human GUCA1A.
The full original mouse double-knockout study was read. Added recombinant GCAP1 restores calcium-sensitive cyclase activity; its construct species was not resolved in the inspected methods. The distinct bovine GCAP2 transgene is not GCAP1.
The target is a membrane-localized calcium sensor in the modeled reaction. The explicit GUCA1A leaf, rather than calcium or a parent entity alone, supplies the compartment assignment.
The target lineage and literal IBD records remain separate from current donor snapshots. Loss checks are snapshot-limited.
The independent evidence file records original source URLs, hashes, target paths and read locators. Cache availability describes the repository copy; it does not replace the recorded original-access scope. Acquisition, validation and subsequent workflow updates are recorded in the curation history.
The current definition separates surrounding outer-segment membrane from internal disc membrane; the queried ancestry has no path between them.
UniProt assigns the location experimentally to PMID:9620085; its lipid-anchor statement is by similarity to bovine GCAP1. Myristoylation does not resolve surrounding versus disc membrane.
Exact human GCAP1โ3 annotations retain regulator/activator terms; inhibitor annotations instead trace the NHERF4 family. The convention remains a curator question; absence of an annotation does not establish absent biological activity.
The new independent records preserve the original Figure4 image, current definitions, exact comparator queries and a zero-hit target GO-CAM index check. No new annotation is proposed.