ACC1 (YNR016C, Q00955) notes

• Cytosolic acetyl-CoA carboxylase, EC 6.4.1.2; single multifunctional polypeptide with BC, BCCP and CT domains [UniProt:Q00955 "Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase"].
• acc1 mutants lack ACC activity and need saturated C14-C16 fatty acids PMID:6103540; acc1-167 is a biotin carboxylase domain lesion PMID:6103540.
• One polypeptide carries both partial activities PMID:6108218.
• Essential even with fatty-acid supplementation (VLCFA / nuclear envelope role) PMID:8098706; PMID:26458104.
• CT domain dimer forms the active site; inhibitors bind there PMID:12663926; PMID:15341732. Holoenzyme is a homodimer PMID:26458104.
• Localisation: cytosol with peripheral association with cytosolic face of nuclear ER PMID:9438137.
• mtr7-1 (acc1) affects Gsp1p nuclear import PMID:12730220 - indirect consequence of altered lipid/nuclear envelope, not a transport function -> over-annotation.
• The mitochondrial ACC is the paralog HFA1, which has an MTS absent from Acc1p PMID:14761959. Mitochondrial HDA/IBA rows for ACC1 therefore questionable.
• "acetyl-CoA biosynthetic process" IMP (PMID:15341732) is wrong direction: ACC consumes acetyl-CoA and makes malonyl-CoA -> MODIFY to malonyl-CoA biosynthetic process.