Gene Ontology annotation through association of InterPro records with GO terms.
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt.
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara.
Automatic assignment of GO terms using logical inference, based on inter-ontology links.
Manual transfer of experimentally-verified manual GO annotation data to homologous complexes by curator judgment of sequence, composition and function similarity
HuCHRAC, a human ISWI chromatin remodelling complex contains hACF1 and two novel histone-fold proteins.
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The paper identifies human CHRAC (HuCHRAC) containing SMARCA5/SNF2H, BAZ1A/ACF1, CHRAC1 (p15), and POLE3 (p17)
"the human homologues of two novel putative histone-fold proteins in Drosophila CHRAC are present in HuCHRAC"
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CHRAC1 and POLE3 form a stable heterodimer that binds naked DNA but not nucleosomes
"two human histone-fold proteins form a stable complex that binds naked DNA but not nucleosomes"
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The complex has ATP-dependent chromatin remodeling activity
"HuCHRAC, a human ISWI chromatin remodelling complex"
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CHRAC1 is a histone-fold protein, not an enzyme
"two novel putative histone-fold proteins in Drosophila CHRAC are present in HuCHRAC"
An ACF1-ISWI chromatin-remodeling complex is required for DNA replication through heterochromatin.
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ACF1-ISWI complex is required for replication through pericentromeric heterochromatin
"an ACF1-ISWI chromatin-remodeling complex is required for replication through heterochromatin in mammalian cells"
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ACF1 and SNF2H become enriched in replicating pericentromeric heterochromatin
"ACF1 (ATP-utilizing chromatin assembly and remodeling factor 1) and an ISWI isoform, SNF2H (sucrose nonfermenting-2 homolog), become specifically enriched in replicating pericentromeric heterochromatin"
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Depletion of ACF1 causes cell cycle delay in late S phase
"depletion of ACF1 causes a delay in cell-cycle progression through the late stages of S phase"
The histone-fold protein complex CHRAC-15/17 enhances nucleosome sliding and assembly mediated by ACF.
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CHRAC-15/17 (CHRAC1/POLE3) facilitates ATP-dependent nucleosome sliding by ACF
"these histone-fold proteins facilitate ATP-dependent nucleosome sliding by ACF"
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Direct interaction of CHRAC-15/17 with ACF1 is essential for nucleosome sliding
"Direct interaction of the CHRAC-15/17 complex with the ACF1 subunit is essential for this process"
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CHRAC-15 (CHRAC1) is essential for interaction with ACF
"CHRAC-15 is essential for interaction with ACF and enhancement of nucleosome sliding"
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CHRAC-17 (POLE3) also interacts with p12 (POLE4) in DNA polymerase epsilon
"CHRAC-17 interacts with another histone-fold protein, p12, in DNA polymerase epsilon"
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CHRAC-15/17 facilitates chromatin assembly by a mechanism different from sliding
"CHRAC-15/17, p12/CHRAC-17, and NC2 complexes facilitate ACF-mediated chromatin assembly by a mechanism different from nucleosome sliding enhancement"
Next-generation sequencing to generate interactome datasets.
A proteome-scale map of the human interactome network.
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
A reference map of the human binary protein interactome.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Deep research review of CHRAC1
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CHRAC1 is a nuclear protein localizing to the nucleoplasm
"CHRAC1 is an intracellular, nuclear protein. It lacks any signal peptides or transmembrane domains, and consistent with its role in chromatin dynamics, it localizes to the cell nucleus, predominantly in the nucleoplasm"
Cyberian deep research on CHRAC1 function