Gene Ontology annotation through association of InterPro records with GO terms

TreeGrafter-generated GO annotations

Combined Automated Annotation using Multiple IEA Methods

tyrB-2 and phhC genes of Pseudomonas putida encode aromatic amino acid aminotransferase isozymes: evidence at the protein level

P. putida aromatic aminotransferase isozymes preferentially transaminate aromatic amino acids and aromatic 2-oxoacids (best substrates L-phenylalanine and phenylpyruvate), using PLP cofactor and 2-oxoglutarate as amino acceptor.

PMID:20050871

Identification and characterization of the PhhR regulon in Pseudomonas putida

Genetic study of aromatic amino acid catabolism in P. putida KT2440; tyrB-1 (PP_1972) and tyrB-2 mutants show altered doubling times on tyrosine and phenylalanine as nitrogen sources, implicating tyrB-family aminotransferases in aromatic amino acid utilization.

PMID:38323821

Machine learning analysis of RB-TnSeq fitness data predicts functional gene modules in Pseudomonas putida KT2440

Disruption of tyrB (PP_1972) did not inhibit growth on L-phenylalanine or L-tyrosine as sole nitrogen sources, whereas disruption of AmaC (PP_3590) abolished growth; the authors propose PP_3590 as the dominant L-tyrosine aminotransferase, indicating PP_1972 is functionally redundant under those conditions.

PMID:35285712

Nitrogen metabolism in Pseudomonas putida: functional analysis using random barcode transposon sequencing

RB-TnSeq fitness data show only weak single-gene fitness effects for PP_1972 on aromatic nitrogen sources, and a PP_3590/PP_1972 double knockout did not cause phenylalanine auxotrophy, consistent with redundancy among aromatic aminotransferases.