Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Schizosaccharomyces pombe mst2+ encodes a MYST family histone acetyltransferase that negatively regulates telomere silencing.
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Mst2 is nuclear and chromatin-bound and negatively regulates telomeric silencing.
"Both proteins are localized to the nucleus and bound to chromatin throughout the cell cycle."
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Mst2 negatively regulates telomeric silencing without measurably changing centromeric or mating-type-region silencing.
"Mst2p is a negative regulator of silencing at the telomere but does not affect silencing in the centromere or mating type region."
Elimination of a specific histone H3K14 acetyltransferase complex bypasses the RNAi pathway to regulate pericentric heterochromatin functions.
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Loss of Mst2 H3K14 acetyltransferase activity bypasses the RNAi requirement for pericentric heterochromatin functions.
"when the activity of the histone H3 Lys 14 (H3K14) acetyltransferase Mst2 is eliminated, the RNAi machinery is no longer required for pericentric heterochromatin functions"
Histone H3 lysine 14 acetylation is required for activation of a DNA damage checkpoint in fission yeast.
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Mst2 is the catalytic subunit of a Mst2 complex that specifically acetylates nucleosomal histone H3K14.
"These results demonstrate that the Mst2 complex is a highly specific nucleosomal histone H3K14 acetyltransferase."
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Mst2 and Gcn5 redundantly acetylate H3K14, and H3K14 acetylation loss in gcn5Δ mst2Δ or H3K14R backgrounds impairs G2-M DNA-damage checkpoint activation.
"Neither gcn5 Δ mst2 Δ nor H3K14R cells show a significant reduction of the septation index upon growth in MMS or after UV treatment, indicating a failure of checkpoint-induced cell cycle delay"
Rapid epigenetic adaptation to uncontrolled heterochromatin spreading.
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The Mst2 complex restrains heterochromatin spreading by regulating histone turnover at heterochromatin regions.
"In this study, we show that Mst2 regulates histone turnover at heterochromatin regions and that loss of Mst2 results in heterochromatin spreading at telomeres and heterochromatin islands where boundaries are absent."
Mapping the dynamics of epigenetic adaptation in S. pombe during heterochromatin misregulation.
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The heterochromatin misregulation system depletes the two major H3K9me antagonists, Mst2 and Epe1, to elicit adaptive Clr4 silencing.
"Deleting two major H3K9me antagonists – the H3K14 histone acetyltransferase Mst2 and the putative H3K9 demethylase Epe1 – leads to the adaptive silencing of the sole H3K9 methyltransferase, Clr4"
Nucleosome remodeler exclusion by histone deacetylation enforces heterochromatic silencing and epigenetic inheritance.
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Artificially targeting Mst2 to H3K9me-marked domains raises H3K14ac and destabilizes heterochromatin through SWI/SNF-family remodelers.
"targeting of chromodomain-fused Mst2 HAT to H3K9me-coated domains increases H3K14ac and SWI/SNF-dependent destabilization of heterochromatin."
An H3K14ub-H3K9me3 feedback circuit governs heterochromatin spreading and inheritance in fission yeast.
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Mst2-mediated H3K14 acetylation opposes the H3K14ub-H3K9me3 feedback circuit to restrain heterochromatin expansion.
"Together, these findings demonstrate that Mst2 and Epe1 function as independent brakes that restrain the H3K14ub-H3K9me3 feedback loop and prevent uncontrolled heterochromatin expansion."
PTHR10615 PAINT IBD export
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PTN004172926 carries broad eukaryotic MYST-family IBD assertions, while fungal node PTN008308138 carries a NuA3a-specific complex assertion seeded by budding-yeast SAS3.
"PTHR10615 PTN008308138 GO:1990467 C IBD false SGD:S000000148 taxon:4751 20230926"