Gene Ontology annotation through association of InterPro records with GO terms

Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features

PMID:24689960

Structural insight into how Streptomyces coelicolor maltosyl transferase GlgE binds α-maltose 1-phosphate and forms a maltosyl-enzyme intermediate.

Biochemical and structural work on Streptomyces GlgE establishes the EC 2.4.99.16 maltose 1-phosphate-dependent maltosyltransferase reaction and double-displacement mechanism conserved by the Pseudomonas ortholog.

"GlgE (EC 2.4.99.16) is an α-maltose 1-phosphate:(1→4)-α-d-glucan 4-α-d-maltosyltransferase of the CAZy glycoside hydrolase 13_3 family."

file:PSEPK/glgE/glgE-uniprot.txt

UniProtKB entry for glgE (Q88FM9)

The unreviewed HAMAP record assigns GlgE EC 2.4.99.16/RHEA:42692, identifies alpha-maltose 1-phosphate binding residues, and describes extension of alpha-1,4-glucan in the TreS/Mak/GlgE/GlgB route.

file:PSEPK/glgE/glgE-deep-research-openscientist.md

OpenScientist functional-annotation report for glgE

The report corroborates the maltose-1-phosphate donor, alpha-1,4-glucan acceptor, GH13 transfer mechanism, and TreS-Mak-GlgE-GlgB pathway placement while finding no direct assay of Q88FM9.

file:interpro/panther/panther-members.tsv

Canonical PANTHER member index

Q88FM9 and the experimentally characterized PAINT seed P9WQ17 are both verified members of PTHR47786:SF2.

"Q88FM9 PTHR47786:SF2"

file:interpro/panther/PTHR47786/PTHR47786-paint.tsv

PANTHER PAINT annotations for PTHR47786

The bacterial ancestral node PTN002233589 carries GO:0016758 with IBD evidence seeded by Mycobacterium tuberculosis GlgE P9WQ17.