Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on curation of immunofluorescence data
Combined Automated Annotation using Multiple IEA Methods
Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function.
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Affinity-enrichment MS interaction mapping validated C17orf89 (NDUFAF8) as a Complex I assembly factor whose disruption markedly reduces Complex I activity and whose depletion is found in an unresolved case of isolated Complex I deficiency.
"we validated C17orf89 as a complex I (CI) assembly factor. Disruption of C17orf89 markedly reduced CI activity, and its depletion is found in an unresolved case of CI deficiency."
Architecture of the human interactome defines protein communities and disease networks.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
A two-step mitochondrial import pathway couples the disulfide relay with matrix complex I biogenesis.
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NDUFAF8 reaches the matrix via a two-step import pathway (weak MTS + TIM23) that exposes it to the IMS disulfide relay for oxidation; in the matrix it binds and stabilizes NDUFAF5, and its loss causes NDUFAF5 degradation, stalled Q-module assembly, and isolated Complex I deficiency.
"NDUFAF8 interacts with its partner NDUFAF5 to stabilize and activate it"