Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Characterization of a rhodanese from the cyanogenic bacterium Pseudomonas aeruginosa.
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Recombinant RhdA transfers sulfur from thiosulfate to cyanide via persulfuration of Cys230; Km thiosulfate 1.0 mM at pH 7.3, Km cyanide 14 mM, Vmax 750 umol/min/mg.
"the titration of active Pa RhdA with cyanide indicates the presence of one extra sulfur bound to the Cys230 Sgamma atom per active enzyme molecule."
Involvement of Pseudomonas aeruginosa rhodanese in protection from cyanide toxicity.
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RhdA is the main cytoplasmic rhodanese (about 90% of activity); the deletion mutant keeps 12% of cytoplasmic activity and grows poorly under cyanogenic conditions.
"The growth of a Δ rhdA mutant is impaired under cyanogenic conditions and fully restored upon complementation with rhdA ."