Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Divalent interaction of the GGAs with the Rabaptin-5-Rabex-5 complex.
Identification of a novel domain in two mammalian inositol-polyphosphate 5-phosphatases that mediates membrane ruffle localization. The inositol 5-phosphatase skip localizes to the endoplasmic reticulum and translocates to membrane ruffles following epidermal growth factor stimulation.
Rabaptin-5alpha/rabaptin-4 serves as a linker between rab4 and gamma(1)-adaptin in membrane recycling from endosomes.
Crystal structure of the clathrin adaptor protein 1 core.
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Gamma is a large structural subunit of the AP-1 tetrameric core.
"The AP-1 core comprises
N-terminal fragments of the two large chains, beta1 and gamma, and the intact
medium and small chains, micro1 and sigma1."
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Gamma-chain mutations impair TGN recruitment; cargo destination is not the same as coat residence.
"directed mutations of residues at a
particular corner of the gamma chain prevent recruitment to the TGN in cells"
AP-1 and AP-3 facilitate lysosomal targeting of Batten disease protein CLN3 via its dileucine motif.
The aftiphilin/p200/gamma-synergin complex.
Functions of adaptor protein (AP)-3 and AP-1 in tyrosinase sorting from endosomes to melanosomes.
Localization to mature melanosomes by virtue of cytoplasmic dileucine motifs is required for human OCA2 function.
AP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis.
Defining the membrane proteome of NK cells.
BLOC-2, AP-3, and AP-1 proteins function in concert with Rab38 and Rab32 proteins to mediate protein trafficking to lysosome-related organelles.
Cell type-specific Rab32 and Rab38 cooperate with the ubiquitous lysosome biogenesis machinery to synthesize specialized lysosome-related organelles.
LAMP1/CD107a is required for efficient perforin delivery to lytic granules and NK-cell cytotoxicity.
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AP-1 perturbation disrupts perforin delivery and target-cell lysis; the external gamma-specific Results resolve the target but not a direct granule-release assay.
"Disruption of expression of LAMP1 binding partner, adaptor protein 1
(AP-1) sorting complex, also causes retention of perforin in the transport
vesicles and inhibits cytotoxicity"
A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Role of the epithelial cell-specific clathrin adaptor complex AP-1B in cell polarity.
Restricted Location of PSEN2/γ-Secretase Determines Substrate Specificity and Generates an Intracellular Aβ Pool.
An interactome perturbation framework prioritizes damaging missense mutations for developmental disorders.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Identification and characterization of novel clathrin adaptor-related proteins.
Degradation of MHC I Complex
Formation of MHC I:Nef:AP-1:PACS-1 Complex
Transport of MHC I:Nef:AP-1:PACS-1 Complex
TGN-lysosomal vesicle coat assembly
Translocation of TGN-lysosome vesicle to lysosome
TGN-lysosome vesicle uncoating and release of nonameric complex to lysosome
trans-Golgi Network Coat Assembly
Vamp And trans-Golgi Network AP-1 Binding Coupled With Cargo Capture
trans-Golgi Network Vesicle Scission
trans-Golgi Network Derived Vesicle Uncoating
trans-Golgi Network Derived Lysosomal Vesicle Uncoating
trans-Golgi Network Lysosome Vesicle Destined Membrane Coat Assembly
trans-Golgi Network Lysosomal Vesicle Scission
Vamp And trans-Golgi Network AP-1 Binding Coupled With Cargo Capture On Lysosome Vesicle Destined Golgi Membrane
CLAT:AP1:CLVS bind PI(3,5)P2
Dissociation of Arf1:GDP, AP-1 Clathrin coated nonameric complex
The gamma/sigma1 and alpha/sigma2 hemicomplexes of clathrin adaptors AP-1 and AP-2 harbor the dileucine recognition site.
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Gamma/sigma hemicomplexes recognize dileucine cargo motifs; human gamma construct evidence is independently established in the external Methods.
"the gamma/sigma1 or alpha/sigma2 hemicomplexes bound the dileucine-based
motifs of several proteins quite strongly"
Structural basis for recruitment and activation of the AP-1 clathrin adaptor complex by Arf1.
Gamma subunit of the AP-1 adaptor complex binds clathrin: implications for cooperative binding in coated vesicle assembly.
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Mouse gamma hinge and appendage cooperate in clathrin recruitment and polymerization in the directly inspected original assay context.
"the hinge and
appendage domains of gamma function in a cooperative manner to recruit and
polymerize clathrin"
Structural basis for the accessory protein recruitment by the gamma-adaptin ear domain.
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Human gamma ear structure-guided mutants identify an accessory-protein recruitment surface.
"Structure-based mutational analyses reveal a binding
site for the accessory proteins that is composed of conserved basic residues"
De novo and bi-allelic variants in AP1G1 cause neurodevelopmental disorder with developmental delay, intellectual disability, and epilepsy.
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Tested recessive AP1G1 variants alter endosomal recycling without an apparent subunit-association defect.
"Functional studies of the
recessively inherited missense variants revealed no apparent impact on the
interaction of AP1γ1 with other subunits of the AP-1 complex but rather showed
to affect the endosome recycling pathway."
AP1G1 manual primary-source research and scope notes