VPS37B PN Consistency Notes

• Generated: 2026-06-18
• Project: PROTEOSTASIS
• Scope: PN consistency rereview against local AIGR review and available deep-research artifacts
• UniProt: Q9H9H4
• AIGR review status: COMPLETE
• Review batch: proteostasis-pr-1217 (PR 1217)
• Batch change status: added

Source Files Checked

• AIGR review YAML: present - genes/human/VPS37B/VPS37B-ai-review.yaml
• AIGR review HTML: present - genes/human/VPS37B/VPS37B-ai-review.html
• Gene notes: present - genes/human/VPS37B/VPS37B-notes.md
• GOA TSV: present - genes/human/VPS37B/VPS37B-goa.tsv
• UniProt record: present - genes/human/VPS37B/VPS37B-uniprot.txt
• PN dossier: projects/PROTEOSTASIS/reports/phase1_dossiers/VPS37B.md
• PN consistency section: projects/PROTEOSTASIS/reports/phase1_dossiers/_sections/VPS37B.md
• PN projection report: projects/PROTEOSTASIS/reports/pn_projection/pn_projected_gene_go_summary.tsv
• PN mapping audit: projects/PROTEOSTASIS/reports/pn_mapping_audit/current_mapping_scrutiny.tsv

Deep Research Files

• No *-deep-research*.md file found in this gene directory.

AIGR Review Snapshot

• Description: VPS37B is a VPS37-family ESCRT-I subunit that complexes with TSG101, VPS28, and MVB12/UBAP-family subunits. It supports ESCRT-I-dependent endosomal sorting of ubiquitinated cargo into multivesicular bodies and contributes to ESCRT-I membrane-remodeling contexts. Current local evidence does not support transferring the VPS37A-specific phagophore-closure role to VPS37B as a core annotation.
• Existing/core annotation action counts: ACCEPT: 24; KEEP_AS_NON_CORE: 11; MARK_AS_OVER_ANNOTATED: 13; MODIFY: 2

PN Consistency Summary

• Consistency: PARTIAL CONTRADICTION. ESCRT-I membership is fully consistent (review ACCEPTs GO:0000813 across many evidence lines; deep notes confirm TSG101/VPS28 co-IP, PMID:15218037; structural headpiece PMID:32424346). But on autophagy the review and PN diverge: the review MARK_AS_OVER_ANNOTATED the GO:0016236 macroautophagy rows and does NOT add GO:0000045, explicitly stating the phagophore-closure role is VPS37A-specific and should not be transferred to VPS37B. PN nonetheless projects GO:0000045 to VPS37B via the shared sealing group.
• PN story / NEW pressure: Over-reaches for autophagy. PN's group-level GO:0000045 projection asserts an autophagosome-assembly role the review judges unsupported for VPS37B (no gene-specific phagophore-closure data; only "probably autophagy" review-level statements). ESCRT-I (GO:0000813) is correctly already captured. No defensible NEW term beyond GO:0000813.
• Evidence alignment: Partial. PN cites only PMID:32424346 (ESCRT-I scaffold), which the review uses for complex membership but explicitly notes used a VPS37B headpiece for general mechanism, not VPS37B autophagy. Review adds PMID:15218037, 23924735 (ALG-2) not in PN.
• Verdict: Consistent on ESCRT-I; GO:0000045 projection over-reaches. Recommended edits: none to YAML (review correctly omits GO:0000045); flag PN sealing-group→GO:0000045 as non-propagating to VPS37B.

Full Consistency Review

• UniProt: Q9H9H4 · batch: proteostasis-pr-1217 · review status: COMPLETE
• PN placement: 1 row, ALP — Autophagosome closure maturation and lysosome fusion → Sealing of autophagophore membrane → ESCRT-I complex component. PN-node mapping: leaf=mapped→GO:0000813 ESCRT I complex; sealing group=mapped→GO:0000045 autophagosome assembly; class=context_only (GO:0016236). Projected: GO:0000045 (more_specific_than_existing_goa), GO:0000813 (already_in_goa_exact).
• Consistency: PARTIAL CONTRADICTION. ESCRT-I membership is fully consistent (review ACCEPTs GO:0000813 across many evidence lines; deep notes confirm TSG101/VPS28 co-IP, PMID:15218037; structural headpiece PMID:32424346). But on autophagy the review and PN diverge: the review MARK_AS_OVER_ANNOTATED the GO:0016236 macroautophagy rows and does NOT add GO:0000045, explicitly stating the phagophore-closure role is VPS37A-specific and should not be transferred to VPS37B. PN nonetheless projects GO:0000045 to VPS37B via the shared sealing group.
• PN story / NEW pressure: Over-reaches for autophagy. PN's group-level GO:0000045 projection asserts an autophagosome-assembly role the review judges unsupported for VPS37B (no gene-specific phagophore-closure data; only "probably autophagy" review-level statements). ESCRT-I (GO:0000813) is correctly already captured. No defensible NEW term beyond GO:0000813.
• Mapping strategy: Flag. The leaf→GO:0000813 mapping is right and harmless (already in GOA). The group→GO:0000045 projection is the issue: it is broader than the review supports for this paralog, paralleling the rejected TOMM20/HSPA8/RAB7A "shared-node over-reach" precedent. GO:0000045 should not propagate to VPS37B on current evidence — restrict the sealing-group projection or mark VPS37B's group membership as non-propagating.
• Evidence alignment: Partial. PN cites only PMID:32424346 (ESCRT-I scaffold), which the review uses for complex membership but explicitly notes used a VPS37B headpiece for general mechanism, not VPS37B autophagy. Review adds PMID:15218037, 23924735 (ALG-2) not in PN.
• Verdict: Consistent on ESCRT-I; GO:0000045 projection over-reaches. Recommended edits: none to YAML (review correctly omits GO:0000045); flag PN sealing-group→GO:0000045 as non-propagating to VPS37B.

PN Dossier Context

• review_batch: proteostasis-pr-1217
• review_yaml: genes/human/VPS37B/VPS37B-ai-review.yaml
• PN workbook rows: 1

PN row 1: Autophagy-Lysosome Pathway | Autophagosome closure maturation and lysosome fusion | Sealing of autophagophore membrane | ESCRT-I complex component

• UniProt: Q9H9H4
• In branches: ALP
• Notes: Component of the ESCRT-I complex, involved in autophagosome closure
• PN references (titles):
  • A helical assembly of human ESCRT-I scaffolds reverse-topology membrane scission | Nature Structural & Molecular Biology
• PN-node mapping records (path + ancestors):
  • [type] Autophagy-Lysosome Pathway|Autophagosome closure maturation and lysosome fusion|Sealing of autophagophore membrane|ESCRT-I complex component
    status=mapped scope=ok_for_propagation_to_go GO=[GO:0000813 ESCRT I complex]
    rationale: This leaf is restricted to ESCRT-I components used in autophagophore sealing. The shared GO assertion is ESCRT I complex membership.
  • [group] Autophagy-Lysosome Pathway|Autophagosome closure maturation and lysosome fusion|Sealing of autophagophore membrane
    status=mapped scope=ok_for_propagation_to_go GO=[GO:0000045 autophagosome assembly]
    rationale: This group captures autophagophore closure/sealing, a late step in autophagosome assembly. Autophagosome assembly is the safer process target than autophagosome-lysosome fusion.
  • [class] Autophagy-Lysosome Pathway|Autophagosome closure maturation and lysosome fusion
    status=context_only scope=too_broad_to_propagate GO=[GO:0016236 macroautophagy]
    rationale: This class is a late macroautophagy context, but the subtree mixes docking, fusion, localization, membrane-composition, and unknown late-stage roles. The class-level relation is useful for display while propagation is restricted to narrower mechanism nodes.
  • [branch] Autophagy-Lysosome Pathway
    status=no_mapping scope= GO=[]
    rationale: Reviewed as the top-level PN branch. It is a project taxonomy umbrella rather than a direct GO assertion; all propagation must come from manually curated child nodes.

Projected GO annotations (2)

• GO:0000045 autophagosome assembly | scope=ok_for_propagation_to_go | goa_status=more_specific_than_existing_goa | from=Autophagy-Lysosome Pathway|Autophagosome closure maturation and lysosome fusion|Sealing of autophagophore membrane
• GO:0000813 ESCRT I complex | scope=ok_for_propagation_to_go | goa_status=already_in_goa_exact | from=Autophagy-Lysosome Pathway|Autophagosome closure maturation and lysosome fusion|Sealing of autophagophore membrane|ESCRT-I complex component

Note

This file is generated from the current PROTEOSTASIS phase-1 dossier and local gene-review artifacts. Edit the source review, PN mapping, or dossier rather than this generated note when correcting the underlying curation.