Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic Gene Ontology annotation based on Rhea mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
ATP and phosphate reciprocally affect subunit association of human recombinant High Km 5'-nucleotidase. Role for the C-terminal polyglutamic acid tract in subunit association and catalytic activity.
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Human recombinant High Km (IMP-specific) 5'-nucleotidase (EC 3.1.3.5) is activated by ATP and inhibited by inorganic phosphate; native enzyme is oligomeric and the C-terminal polyglutamic/aspartic tail is required for subunit association.
"IMP-specific, High Km 5'-nucleotidase (EC 3.1.3.5) is an ubiquitous enzyme, the activity of which is highly regulated by substrate, ATP, and inorganic phosphate."
Cytosolic and mitochondrial deoxyribonucleotidases: activity with substrate analogs, inhibitors and implications for therapy.
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cN-II dephosphorylates the monophosphates of purine nucleoside analogs, and its overproduction can lead to resistance against purine analog drugs.
"overproduction of cN-II could lead to resistance against purine analogs"
Towards a proteome-scale map of the human protein-protein interaction network.
Nucleoside phosphotransferase activity of human colon carcinoma cytosolic 5'-nucleotidase.
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A human cytosolic 5'-nucleotidase acting preferentially on IMP and GMP also transfers the phosphate of 5'-NMPs to nucleosides (phosphotransferase activity), and is activated by ATP and 2,3-diphosphoglycerate.
"A cytosolic 5'-nucleotidase, acting preferentially on IMP and GMP, has been"
Crystal structure of human cytosolic 5'-nucleotidase II: insights into allosteric regulation and substrate recognition.
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cN-II dephosphorylates 6-hydroxypurine nucleoside 5'-monophosphates, regulates IMP and GMP pools, is a homotetramer with two allosteric effector sites, and is activated by adenine-based nucleotides and 2,3-bisphosphoglycerate.
"Cytosolic 5'-nucleotidase II catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates and regulates the IMP and GMP pools within the cell."
An empirical framework for binary interactome mapping.
Structural basis for the allosteric regulation and substrate recognition of human cytosolic 5'-nucleotidase II.
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Seven high-resolution structures reveal that ATP, diadenosine polyphosphates and 2,3-bisphosphoglycerate act as allosteric activators binding effector sites, with an effector-induced disorder-to-order transition coordinating catalytic Mg2+.
"It is allosterically activated by a number of phosphate-containing cellular metabolites such as ATP, diadenosine polyphosphates, and 2,3-bisphosphoglycerate"
Suppression of 5'-nucleotidase enzymes promotes AMP-activated protein kinase (AMPK) phosphorylation and metabolism in human and mouse skeletal muscle.
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siRNA silencing of NT5C2 in human myotubes reduced cytoplasmic 5'-nucleotidase activity and raised the AMP/ATP ratio, activating AMPK; NT5C2 is an IMP-preferring cytosolic 5'-nucleotidase.
"Using siRNA to silence NT5C2 expression in cultured human myotubes, we observed a 2-fold increase in the AMP/ATP ratio"
A proteome-scale map of the human interactome network.
Architecture of the human interactome defines protein communities and disease networks.
A reference map of the human binary protein interactome.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
TRIM22 orchestrates the proliferation of GBMs and the benefits of TMZ by coordinating the modification and degradation of RIG-I.
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In glioblastoma cells NT5C2 forms a complex with TRIM22 and is reported to be responsible for K48-linked ubiquitination of RIG-I, negatively regulating RIG-I/NF-kB antiviral signaling; the NT5C2-RIG-I interaction is indirect.
"NT5C2 is responsible for K48-linked ubiquitination"
Molecular cloning of human cytosolic purine 5'-nucleotidase.
Bovine cytosolic IMP/GMP-specific 5'-nucleotidase: cloning and expression of active enzyme in Escherichia coli.
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Recombinant IMP/GMP-specific cytosolic 5'-nucleotidase (99.5% identical to human NT5C2) exhibits both 5'-nucleotidase and phosphotransferase activity; enzyme is cytosolic and widely expressed.
"It exhibited both 5'-nucleotidase and phosphotransferase activity"
carbovir + IMP => carbovir monophosphate + inosine
(d)GMP or (d)IMP + H2O => (2'-deoxy)guanosine or (2'-deoxy)inosine + orthophosphate (NT5C2)
NT5C2 tetramer phosphorylates RBV
NT5C2 tetramer dephosphorylates RBV-MP