GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000118

TreeGrafter-generated GO annotations

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

file:PSEPK/mvaB/mvaB-uniprot.txt

UniProtKB entry Q88H25 for Pseudomonas putida KT2440 mvaB

UniProt identifies Q88H25 as hydroxymethylglutaryl-CoA lyase and records the HMG-CoA cleavage reaction.

"RecName: Full=hydroxymethylglutaryl-CoA lyase"

PMID:16330546

Crystal structures of two bacterial 3-hydroxy-3-methylglutaryl-CoA lyases suggest a common catalytic mechanism among a family of TIM barrel metalloenzymes cleaving carbon-carbon bonds.

Structures of two bacterial HMG-CoA lyases identify a conserved divalent-cation site in the catalytic center.

"the catalytic center contains a divalent cation-binding site formed by a cluster of invariant residues that cap the core of the barrel"

PMID:19459965

The Pseudomonas aeruginosa liuE gene encodes the 3-hydroxy-3-methylglutaryl coenzyme A lyase, involved in leucine and acyclic terpene catabolism.

The close Pseudomonas LiuE ortholog was purified and shown to cleave HMG-CoA to acetyl-CoA and acetoacetate.

"cleavage of HMG-CoA to acetyl-CoA and acetoacetate."

PMID:16820476

Identification of genes and proteins necessary for catabolism of acyclic terpenes and leucine/isovalerate in Pseudomonas aeruginosa.

P. putida was observed to utilize leucine and isovalerate but not acyclic terpenes.

"while both species were able to utilize leucine and isovalerate."

file:PSEPK/mvaB/mvaB-deep-research-openscientist.md

OpenScientist gene research for PSEPK mvaB

The report supports MvaB as the principal HMG-CoA lyase candidate while retaining uncertainty about the PP_3394 paralog and direct KT2440 biochemistry.

"No direct biochemistry on PP_3540 itself."
The provider reports high sequence identity between MvaB and characterized P. aeruginosa LiuE.

"| mvaB (PP_3540) vs. *P. aeruginosa* LiuE/PA2011 (characterized HMG-CoA lyase) | **78.6%** |"
The provider reports that mvaB is genomically separated from the clustered upstream leucine-catabolism genes.

"By contrast, **mvaB/PP_3540 is located ~500 genes away** from this cluster"