Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
A micropeptide encoded by a putative long noncoding RNA regulates muscle performance.
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A 46-aa conserved micropeptide (MLN) is translated from the human LINC00948 / mouse AK009351 transcript.
"we identified a vertebrate RNA transcript annotated as a lncRNA (LINC00948 in humans and AK009351 in mice)"
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MLN co-immunoprecipitates with SERCA1, SERCA2a and SERCA2b, and hydrophobic TM residues shared with PLN/SLN are required.
"the HA-MLN fusion protein formed a stable complex with SERCA1 (skeletal muscle-specific), SERCA2a (cardiac and slow skeletal muscle-specific) and SERCA2b (ubiquitous) isoforms"
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MLN knockout mice have enhanced SR Ca2+ handling and exercise performance.
"Remarkably, MLN KO mice ran an average time of ∼31% longer than their WT littermates, representing a 55% increase in running distance (Figure 6E and 6F)."
Widespread control of calcium signaling by a family of SERCA-inhibiting micropeptides.
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MLN, PLN, SLN, ALN and ELN form a family of SERCA-inhibiting micropeptides with isoform-matched expression patterns.
"We note a remarkable overlap in the distribution of individual micropeptides with major SERCA isoforms (SERCA1 with MLN, SERCA2a with PLN, SERCA2b with ALN, and SERCA3 with ELN)"
Newly Discovered Micropeptide Regulators of SERCA Form Oligomers but Bind to the Pump as Monomers.
Nothing Regular about the Regulins: Distinct Functional Properties of SERCA Transmembrane Peptide Regulatory Subunits.
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In co-reconstitution with SERCA1a, MLN lowers Vmax without altering KCa.
"Using a membrane reconstitution system, we have found that MLN selectively alters the Vmax of SERCA with no effect on the KCa of SERCA (Figure 5B and Table 1)."
Functional Role of an Unusual Transmembrane Acidic Residue in the Calcium Pump Regulator Myoregulin.
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The TM residue Asp35 (conserved in human) controls SERCA inhibition by MLN.
"Instead, Asp35 controls SERCA inhibition by populating a bound-like orientation of MLN."
Replacement of Lys27 by asparagine in the SERCA regulator myoregulin: A Ca(2+) affinity modulator or a catalytic activity switch?
NR1D1 controls skeletal muscle calcium homeostasis through myoregulin repression.
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In human immortalized myotubes, MLN overexpression lowers and MLN shRNA restores SR Ca2+ content; NR1D1 represses MLN transcription.
"Whereas MLN overexpression in healthy myotubes reduced SR calcium content (Supplemental Figure 2, I and J), shRNA against MLN improved SR calcium content in DMD immortalized myotubes (Supplemental Figure 2, K and L)."
Canonical Wnt signaling affects calcium homeostasis in serum-treated AC16 cells through MLN-mediated SERCA2a regulation.
Early vertebrate origin and diversification of small transmembrane regulators of cellular ion transport.
Cardiac effects of myoregulin in ischemia-reperfusion.
UniProt entry P0DMT0 (MLN_HUMAN)