CG34117 (Q0KI97) notes

2026-10-21 — Full re-review, independent of the ProtNLM2 predictions

No ProtNLM2 file was opened or used in this re-review.

Q0KI97 is the only polypeptide of CG34117 (FBgn0083953). FlyBase reports one transcript and one
unique polypeptide. The sequence is 101 aa, sequence version 1, UniProt TrEMBL, PE 3.
It is not a fragment, isoform or alternative gene model. It aligns collinearly over its whole length
with human FMC1 (Q96HJ9, 113 aa), at 40.6% identity over 101 positions, and carries the conserved
C-terminal block (HGxGERSV...LVGxKLP). See CG34117-bioinformatics/RESULTS-rereview.md.

CG34117 and human FMC1 are both in PANTHER PTHR31716:SF1. The PAINT node is PTN001271866.
Yeast Fmc1 (P40491) is in PANTHER PTHR28015, a different family. Human FMC1 was characterized
in PMID:28719601.
The animal and yeast proteins share only the Pfam Complex1_LYR_2 assignment.
The previous version of this review used yeast Fmc1 as its main support. The re-review rebuilds the
support on human FMC1:
mitochondrion: PMID:28719601
complex V assembly by knockout and rescue: PMID:28719601
binding to ATPAF2: PMID:28719601

GO:0003674 ND: ACCEPT. There is no informative MF, and ATPAF2 binding would map only to bare
protein binding.
GO:0005739 IBA: ACCEPT. The donor is in-subfamily, with IDA evidence.
GO:0005759 ISS (with P40491): changed from ACCEPT to UNDECIDED. The donor is from another
family. Matrix localization is plausible because FMC1 binds ATPAF2, which works on the matrix side,
but no PTHR31716 member has been placed in the matrix. A WRONG_WITH_FROM source defect (CHECK)
was recorded for FlyBase.
GO:0033615 IBA and ISS (both with Q96HJ9): ACCEPT. These form the core function.
No NEW terms were added.

interpro/panther/PTHR31716/PTHR31716-review.yaml describes the family mainly through yeast Fmc1
(PMID:11096112). That conflicts with PANTHER placing yeast Fmc1 in PTHR28015, and the review does
not cite the human FMC1 experimental paper (PMID:28719601). There is no disagreement about subfamily
placement: CG34117 is in SF1, as is human FMC1.
Module: modules/dmel_respiratory_complex_assembly_factors.yaml (part complex_v_assembly_cg34117).

Human FMC1 (Q96HJ9) is the in-subfamily ortholog and is experimentally characterized. The repo has
no genes/human/FMC1 review.