Gene Ontology annotation through association of InterPro records with GO terms
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
Glypican-3 binds to Frizzled and plays a direct role in the stimulation of canonical Wnt signaling.
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GPC3 promotes canonical Wnt signaling.
"GPC3 stimulates HCC growth by promoting canonical Wnt signaling."
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GPC3 binds Frizzled directly through its glycosaminoglycan chains.
"GPC3 and Frizzled interact directly through the glycosaminoglycan chains of GPC3"
Processing by proprotein convertases is required for glypican-3 modulation of cell survival, Wnt signaling, and gastrulation movements.
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Furin-like processing of GPC3 is needed for Wnt modulation and embryonic cell movements.
"This processing is essential for GPC3 modulating Wnt"
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In transfected CHO cells, GPC3 repressed Wnt1-induced canonical reporter activity, showing that the sign of its effect on canonical Wnt depends on context.
"increasing the amount of wild-type GPC3 repressed Wnt1-mediated pTOPFLASH activation in a dose-dependent manner"
Glypican-3 inhibits Hedgehog signaling during development by competing with patched for Hedgehog binding.
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Mouse GPC3 binds hedgehog with high affinity, competes with Patched, and induces hedgehog endocytosis and degradation; heparan sulfate chains are not needed for the interaction.
"GPC3 competes with Patched for Hedgehog binding"
Overgrowth of a mouse model of Simpson-Golabi-Behmel syndrome is partly mediated by Indian hedgehog.
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Overgrowth of Gpc3-null mice is partly rescued by loss of Ihh, which places GPC3 upstream of hedgehog-driven growth in vivo.
"the developmental overgrowth observed in Gpc3-null mice is, at least in part, a consequence of the hyperactivation of the Hh pathway"
UniProt record for donkey GPC3 (A0A9L0J2B1)
UniProt record for human GPC3 (P51654)
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Human GPC3 is a GPI-anchored cell surface proteoglycan.
"Cell membrane {ECO:0000269|PubMed:14610063}; Lipid-anchor, GPI-anchor"
GPC3: donkey-human sequence comparison
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Processing, glycosaminoglycan and GPI-anchor sites are conserved in donkey GPC3.
"The furin-like cleavage site (human R358/S359), the two heparan sulfate attachment serines (human S495, S509) and the GPI-anchor asparagine (human N554) are all conserved in the donkey sequence."
GPC3 (donkey A0A9L0J2B1): N-terminal gene-model check (re-review, 2026-10-21)
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A0A9L0J2B1 lacks the human signal peptide, so its N-terminus comes from an alternative first exon. The rest of the protein is 96% identical to human GPC3.
"A0A9L0J2B1 is therefore an alternative-first-exon gene model of donkey GPC3, not the canonical product"
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The canonical donkey GPC3 product is A0A9L0JGR2.
"The canonical donkey GPC3 product is A0A9L0JGR2."