Gene Ontology annotation through association of InterPro records with GO terms.
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt.
Automatic Gene Ontology annotation based on Rhea mapping.
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods.
Global analysis of protein localization in budding yeast.
Neuropathy target esterase and its yeast homologue degrade phosphatidylcholine to glycerophosphocholine in living cells.
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Yeast Nte1/YML059c is an ER-localized neuropathy-target-esterase homolog required for phosphatidylcholine deacylation to glycerophosphocholine.
"YML059c, a Saccharomyces cerevisiae protein with sequence homology to NTE, had similar catalytic properties to the mammalian enzyme in assays of microsome preparations and, like NTE, was localized to the endoplasmic reticulum. Yeast lacking YML059c were viable under all conditions examined but, unlike the wild-type strain, did not convert PtdCho to GroPCho."
NTE1-encoded phosphatidylcholine phospholipase b regulates transcription of phospholipid biosynthetic genes.
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Nte1-dependent ER phosphatidylcholine deacylation secondarily regulates Opi1-dependent transcription of phospholipid biosynthetic genes.
"The Saccharomyces cerevisiae NTE1 gene encodes an evolutionarily conserved phospholipase B localized to the endoplasmic reticulum (ER) that degrades phosphatidylcholine (PC) generating glycerophosphocholine and free fatty acids. We show here that the activity of NTE1-encoded phospholipase B (Nte1p) prevents the attenuation of transcription of genes encoding enzymes involved in phospholipid synthesis in response to increased rates of PC synthesis by affecting the nuclear localization of the transcriptional repressor Opi1p."
One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy.
Falcon deep research report for NTE1
High confidence proteomic analysis of yeast LDs identifies additional droplet proteins and reveals connections to dolichol synthesis and sterol acetylation.
The acyltransferase Gpc1 is both a target and an effector of the unfolded protein response in Saccharomyces cerevisiae.
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The 2023 Gpc1 study confirms that Nte1- and Plb1-generated glycerophosphocholine is the entry substrate for the Gpc1/Ale1 PC deacylation/reacylation pathway.
"In PC-DRP (Fig. 1), PC undergoes complete deacylation by type B phospholipases, primarily Nte1 and Plb1"