The Tre2 oncoprotein, implicated in Ewing's sarcoma, interacts with two components of the cytoskeleton.
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The Tre2 GAP region binds an ankyrin-repeat protein, confirmed by several methods.
"Two components of the cytoskeleton were thus identified, whose interaction with the GAP region was confirmed by GST-pulldown, co-immunoprecipitation, and colocalisation experiments."
Protein phosphatase 6 regulatory subunits composed of ankyrin repeat domains.
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ANKRD44 co-purifies with PP6 through PPP6R1 in human cells.
"Mass spectrometry identified coprecipitating proteins as PP6 catalytic subunit and three ankyrin repeat proteins (Ankrd28, Ankrd44, and Ankrd52)."
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The three PP6 ankyrin subunits are thought to have distinct functions.
"segregate into separate branches on a phylogenetic tree for vertebrate species, suggesting individual biological functions"
Protein interaction network of the mammalian Hippo pathway reveals mechanisms of kinase-phosphatase interactions.
A proteome-scale map of the human interactome network.
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Multimodal cell maps as a foundation for structural and functional genomics.
Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.
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ANKRD44 depletion alters nuclear morphology.
"Depletion of the ANKRD52 subunit had no effect on nuclear morphology, whereas ANKRD28 and ANKRD44 ankyrin repeat subunit depletion altered nuclear morphology"
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ANKRD28 and ANKRD44 are required for PP6 in mitosis.
"The results presented here suggest that the ANKRD28 and 44 subunits are required for PP6 function in mitosis, whereas ANKRD52 is not."
Protein phosphatase 6 promotes stemness of colorectal cancer cells.
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ANKRD44 is one of three PP6 scaffold subunits.
"PP6 functions as a heterotrimer with a catalytic subunit (PP6c), regulatory subunits (PP6R1, PP6R2, and PP6R3), and scaffold subunits (ANKRD28, ANKRD44, and ANKRD52)."
miR-133a-3p inhibits the osteogenic differentiation of bone marrow mesenchymal stem cells by regulating ankyrin repeat domain 44.
ANKRD44 Gene Silencing: A Putative Role in Trastuzumab Resistance in Her2-Like Breast Cancer.
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ANKRD44 silencing activates NF-kB and causes partial trastuzumab resistance.
"The ANKRD44 gene was silenced in Her2-like breast cancer cell line (BT474), obtaining a partially Trastuzumab-resistant breast cancer cell line that constitutively activates the NF-kb protein via the TAK1/AKT pathway."
UniProt entry Q8N8A2 (ANR44_HUMAN)
Affinage mechanistic annotation for ANKRD44 (human)