FBXO41 review notes

2026-07-14 cerebellum-development audit

• Rechecked all 14 GOA-derived annotations and the two pre-existing proposed
  annotations against the reviewed UniProt record, the Falcon deep-research report,
  full-text PMID:31160656, full-text PMID:26063905, and FBXO41's role in
  modules/cerebellum_development.yaml.
• The module places FBXO41 in the post-specification granule-cell migration program.
  This is supported by the existing proposed GO:0001764 neuron migration annotation:
  cytoplasmic Fbxo41 rescue and gain-of-function experiments alter migration from the
  external granule layer toward the internal granule layer PMID:26063905.
• Kept the 10 Reactome cytosol rows as non-core pathway context. They are legitimate
  machine-sourced rows but do not constitute FBXO41-specific experimental localization;
  the general SCF core function therefore no longer asserts cytosol as its activity
  location.
• Accepted the centrosome annotation as a core functional location because the ortholog
  is experimentally localized to centrioles/centrosomes and this is the site from which
  increased Fbxo41 drives cilium shortening PMID:31160656.
• Added a proposed GO:1990756 ubiquitin-like ligase-substrate adaptor activity
  annotation by orthology-supported inference. The study establishes F-box-dependent
  SKP1/CUL1 assembly, and UniProt describes human FBXO41 as the SCF substrate-recognition
  component. This does not imply that FBXO41 itself catalyzes ubiquitin transfer, and a
  physiological FBXO41 substrate remains unknown PMID:31160656.
• Recorded GO:0019005 SCF ubiquitin ligase complex in in_complex for the synthesized
  core activities rather than treating the complex as a cellular location.
• The cilium-disassembly proposal remains deliberately qualified: increased Fbxo41 is
  sufficient to shorten/disassemble cilia, but knockdown did not alter basal cilium
  length and PDBU can drive disassembly without Fbxo41. The review therefore does not
  claim that FBXO41 is universally required for cilium disassembly or identify an
  unknown ciliary ubiquitination substrate PMID:31160656.