The genuine fetch returned 36 QuickGO source contexts and seeded 35 review rows. The UniProt and ComplexPortal GO:0000139/IDA/PMID:29477842 contexts differ in source attribution and project to one review row. The immutable source TSV retains both. All 35 seeded source objects and their order are preserved; both underlying contexts were reviewed. Stored decisions are 25 ACCEPT and 10 KEEP_AS_NON_CORE; source-context decisions are 26 ACCEPT and 10 KEEP_AS_NON_CORE. There are no NEW annotations, natural alternative products or supporting-text quotations.
The core is ribitol-phosphate transfer to the previously modified, phosphorylated core M3 glycan of alpha-dystroglycan. Human FKRP structural/activity work supports the second-transfer mechanism and functionally relevant protomer pairing PMID:31949166(https://pubmed.ncbi.nlm.nih.gov/31949166/). The broad GO:0016780 accurately describes this chemistry. GO:0047356 was examined, but no replacement is inferred from its ribitol name or teichoate synonyms. The term's polymer reaction needs a separate specificity assessment; no bacterial taxon restriction is asserted. GO:0035269 includes later extension of the O-mannose glycan, which FKRP directly performs.
Three IBAs were retained after matching the original IBD assertions at PTN001007059 to the actual human target path in the complete 102-node PTHR13627 topology. Human experimental seed membership is legitimate grounding. The exact-main family files were preserved, without downloading a bulk leaf GAF.
Endogenous human muscle supports Golgi residence PMID:21886772(https://pubmed.ncbi.nlm.nih.gov/21886772/), but the original rough-ER study also includes endogenous human myotubes and biopsy PMID:15213246(https://pubmed.ncbi.nlm.nih.gov/15213246/). It is not dismissed as solely ectopic or mutant protein. Secretion in CHO cultures remains contextual PMID:19900540(https://pubmed.ncbi.nlm.nih.gov/19900540/). Endogenous mouse sarcolemma evidence supports the existing orthology-based mapping, without asserting direct human surface catalysis PMID:17452335(https://pubmed.ncbi.nlm.nih.gov/17452335/). The HPA Golgi row is accepted as trusted curation plus independent evidence; its images and scoring were not independently inspected.
Species and construct boundaries matter. The inspected localization construct in PMID:25279699 is mouse Fkrp pTW323 in HEK293T; the companion FKTN insert is human. The transfer assays in PMID:27194101 use mouse enzymes and rabbit dystroglycan in human host cells. The soluble 45–495 and Asn33-start human FKRP constructs in PMID:31949166 are engineered, as are the secretion/truncation constructs in other papers. None is promoted to a natural alternative product.
Two supported generic-binding annotations remain non-core under the explicit campaign instruction, which overrides the skill's default removal of uninformative binding. Tagged co-IP and recovered activities do not establish purified binary contacts, fixed stoichiometry, obligatory assembly or channeling. The specific FKRP self-association annotation is supported by the structural/functional evidence. Tetramer formation is retained as non-core because its physiological significance remains unresolved.
All 19 normal sources were obtained: ten were hydrated and verified byte-for-byte from qualified main before the genuine fetch, and nine absent sources were acquired by normal caching. Abstract-only normal caches were not edited to insert separately recovered full text. Primary HTML, scanned original pages, the author summary and their access limitations are documented in the source guide. The known current provider credential/quota failure was not retried, and no provider-named research file was fabricated. Supporting evidence is reference-only, following the instruction to add no publication quotations.
Annotation-reviewer and core-function-synthesizer criteria were applied. Independent peer QA and the validation results will be appended after the candidate is checked.
Independent annotation-reviewer QA read all 35 stored decisions, all 36 original contexts, the single core function and the source boundaries, and reported no concrete issues. The source projection, original fields/order and the two collapsed Golgi provenances were checked independently. Schema/reference/ontology validation, strict GOA comparison, report-only status, explicit-codex history validation and private HTML rendering passed. Two expected generic-binding advisories remain under the campaign instruction; the dependency emitted a pkg_resources deprecation warning. The PMID pathway helper found no pathway markdown and is not claimed as validation of these notes. Private HTML text, expected biological content and evidence links were checked; no pixel inspection was performed. The tracked GO cache and all 19 normal-source files remain unchanged after validation. Final publication still requires coordinator review of the committed object set.
The earlier counts above describe the initial candidate. In response to the review, the two cytoplasm rows now use TOO_GENERAL. The current stored counts are 25 ACCEPT, 8 KEEP_AS_NON_CORE and 2 TOO_GENERAL; all 36 original contexts are covered by 26 ACCEPT, 8 KEEP_AS_NON_CORE and 2 TOO_GENERAL. Fresh official GO paths establish Golgi membrane part_of Golgi apparatus part_of cytoplasm, so the accepted specific locations already entail the broad term. This judges term precision rather than rejecting source evidence, claiming soluble cytosolic catalysis, or excluding additional intracellular pools. The rough-ER, extracellular and sarcolemmal contexts remain non-core.
PMID:29884773 is now explicitly linked to donor specificity in the accepted phosphotransferase reason and a dedicated question. Its soluble-enzyme glycerol-phosphate transfer and inhibition findings do not establish physiological regulation. The existing metal question explicitly records the decision not to propose GO:0000287: Mg and Mn both support activity in vitro, the cellular metal remains unresolved and structural zinc is distinct. No new subcompartment annotation or ontology term is proposed. The single core, standalone description, all 35 seed objects/order, all 36 original contexts, 19 normal sources, zero natural products and zero quotations are preserved. A new explicit-codex history record documents this follow-up without changing the initial record.