YvoF acetylates the glyceryl hydroxyls of HepG using acetyl-CoA. The 2016 study identifies yvoF through loss of native lipid acetylation and characterizes recombinant activity; MAT is a related enzyme with overlapping in vitro scope but fails to rescue the yvoF deletion. This supports lipid O-acetylation as the physiological role, not an additional maltose metabolic function. PMID:27226549
The 2025 study directly connects YvoF-dependent carrier loading with lipoprotein acetylation. Native yvoF deletion changes FrxB N-terminal peptide mass by an acetyl unit and complementation restores it. The biochemical reconstitution uses recombinant YvoF, a geranylgeranyl C20 analogue of the native C35 carrier, calf intestinal alkaline phosphatase and recombinant LhaT-containing E. coli membranes. It does not identify the native phosphatase, establish which monoacetyl regioisomer/diacetyl species LhaT prefers, or demonstrate the membrane-crossing mechanism. PMID:40626731
The 2016 primary localization paragraph, independently accessible through the PMC search index, reports activity in membrane-containing fractions and none in the soluble fraction. Its high-salt extraction and sequence/structure comparison favor peripheral association; the physical tether remains unknown. The 2025 cytoplasmic loading model is compatible with membrane-associated YvoF rather than a freely soluble cytosolic enzyme. Side-of-membrane assignment is mechanistic inference, not a direct topology map. A short excerpt and access provenance are archived in yvoF-source-analysis/PMID27226549-reading.md.
The sole seeded GO:0016407 IBA is MODIFY to GO:0016413 O-acetyltransferase. The generic inherited chemistry is correct; target biochemical evidence supplies acceptor-atom specificity. just paint-losses BACSU yvoF found no IRD/IKR on this lineage. This is not a complete ancestral-node reconstruction and no loss is inferred. No NEW rows are necessary. The plasma-membrane core location reflects functional association, not transmembrane topology.
No TLR2 regulatory or immune-process annotation is added: the enzyme modifies a bacterial lipid carrier; downstream lipoprotein chemotypes are the receptor ligands. Necessity in a reporter screen alone would not establish an immune-signaling activity of YvoF. The 2025 paper's figure alternative text occasionally simplifies mutant results; scientific decisions use the Results and Methods text, not those summaries.
A genuine just deep-research BACSU yvoF --provider falcon attempt failed with HTTP403 authentication. No provider report was fabricated. The full 2025 primary and cached 2016 abstract were read. Direct PMC2016 access returned CAPTCHA, publisher access403, and EuropePMC fullTextXML500; indexed primary Results supplied only the localization passage. These failed responses are not biological evidence, and the publication cache remains unchanged. Logs are in .cache/module-audit-logs/hepg-20261107/new-bacillus-members/.
Official QuickGO definitions were retrieved for acetyltransferase, O-acetyltransferase, N-acetyltransferase and donor-specific N-terminal acetyltransferase terms; responses are archived in the consultation audit directory. The original single annotation payload/order and raw source hashes are retained in the seed baseline.