Large-scale GFP-fusion localization study classifying 75% of yeast proteome into 22 subcellular localization categories. YGR117C-GFP fusion localizes to the cytoplasm.

Proteome-wide protein expression quantification. YGR117C is present at approximately 1280 molecules per cell in log phase SD medium.

Original genomic sequencing of the chromosomal region containing YGR117C. The gene was identified as one of 11 new open reading frames in a 23.4 kb segment on chromosome VII.

YGR117C remains poorly characterized. The strongest gene-specific experimental evidence retrievable links the protein to translation fidelity/termination and overall protein synthesis, based on reporter assays in a YGR117C deletion strain, and cites prior GFP-tag localization placing the protein in the cytoplasm.

In a translation-fidelity follow-up, deletion of YGR117C increased premature stop-codon readthrough in a beta-galactosidase reporter assay, with qRT-PCR controls indicating the effect was at the translation level rather than due to altered reporter mRNA abundance. The authors interpret this as YGR117C being involved in the translation pathway. Evidence is from a 2014 PhD thesis, not a peer-reviewed gene-specific paper.

Using a GAL1-driven beta-galactosidase protein-synthesis assay, YGR117C deletion was among strains showing decreased protein synthesis, again with qRT-PCR indicating mRNA levels did not account for the effect. This provides convergent but still indirect evidence of an effect on translation output in vivo.

YGR117C appears as one of 446 candidate genes in a genome-wide nonstop-decay (NSD) screen, but no gene-specific validation, effect size, or mechanistic interpretation was provided. Falcon concludes that assignment to NSD/autophagy is not currently supported beyond candidate-list inclusion and should be treated as speculative.

The protein product is reported as cytoplasmic based on cited GFP-localization work (Huh et al. 2003), consistent with translation-related roles, though it does not specify association with ribosomes, stress granules, or other subcompartments.

No direct enzymatic activity, substrate specificity, or validated binding partners/complex membership were identified for YGR117C. The LisH/WD40 scaffold-adaptor hypothesis is explicitly flagged as an inference not supported by direct evidence in the retrieved corpus and should not be treated as established function.