This guide separates source measurements, curator inferences and biological synthesis. All 21 original annotation dictionaries and four product identifiers are preserved. The standalone review contains one complex-dependent catalytic core and no NEW annotation.
| Source | Checkable evidence and actual access | Limits |
|---|---|---|
| PMID 10519411 | Separate AACR original indexed sections: Subcellular Fractionation; Subcellular Localization; Figure 4C caption. Human ovarian epithelial fractions and tagged COS-1/HOSE imaging support nuclear, cytoplasmic and perinuclear pools. | Normal cache remains abstract-only. Selected original text, not a complete downloaded original or figure pixels, was read. p70/p85 bands are not assigned to natural isoforms. |
| PMID 25416956 | Actual original Results on two independent screens, pairwise quadruplicate retests and identity confirmation; Experimental Procedures on ORFs and identification. Exact IntAct pair EBI-10456463, EBI-10545280, EBI-10303209. | Protected cache has a full-text flag but lacks decisive Results/Methods; the separate genuine original was reused. No pair-specific orthogonal validation or endogenous physiological consequence is inferred. |
| PMID 30877278 | Recovered main Methods, functional Results/Discussion and Figure 1: human DPH1 expression in deficient MCF7 cells, followed by toxin/biotin-NAD labeling of EEF2 in extracts. | Normal cache is abstract-only. Supplements could not be recovered. The structural model is not an experimentally solved human complex; the assay is not purified human catalysis. |
| PMID 31463593 | Genuine full XML cache; original yeast heterodimer reconstitution, cluster mutants, EPR and Figure 6. Dph1 and Dph2 cluster functions are separated by reductant conditions. | Yeast biochemical inference to human, not autonomous human-subunit activity. Cache bytes are identical across normal fetch, current main and the independently pinned pending copy. |
| HPA DPH1 | Official XML, HPA069750 ICC/IF: A-549 and U2OS nucleoplasm/cell junctions; approved summary reliability and supported subassay. | Annotation records were read; image pixels were not independently scored. Localization is nonexclusive and not product-specific. |
| Reactome 5358494 | Catalyst 5358378 → complex 5358365 → human DPH1 5358384, residues 1–438, cytosol. | The model's DPH2 activeUnit attribution is based on archaeal homology; it is not the basis for assigning the human catalytic subunit. Accessory DPH3 membership does not assert immutable complex stoichiometry. |
| PANTHER PTHR10762 | Current tree target HUMAN/HGNC:3003/Q9BZG8 descends from PTN000079945. The literal IBD is GO:0017183 with yeast, mouse and human descendant evidence. | An IBD is a curated phylogenetic judgment. No inference is rejected from donor count or because DPH1/DPH2 belong to the same ancestral family. |
| InterPro IPR016435 | Current GO:0017183 and GO:0090560 mappings; exact Q9BZG8 target match, residues 25–401 of 438. | Family membership alone does not establish autonomous subunit catalysis. |
| Yeast P40487, mouse Q5NCQ5 | Complete current QuickGO donor records were inspected. P40487 has experimental process, cluster-binding, contribution-qualified activity and synthase-complex evidence. Q5NCQ5 has process, activity, localization and complex records. | Original assays are cited only when actually read through the independent consultation. Donor database rows do not substitute for unread full papers; target qualifiers remain literal. |
The binding-record ROGIDs are hcvtBckAa6QAiPOZppYB8Yl1MTQ9606 (DPH1) and M7mQ08NDYxuawmUEKRgjSdIr+Og9606 (TFCP2). They match the checked parent/reference sequences; this checksum agreement is not independent authentication of an archived experimental ORF. The records have no annotated bait-region feature; no inference about unrecorded tags, all isoforms or native stoichiometry is made. The verified GO:0140297 definition requires a DNA-binding transcription-factor partner, whose class is supported independently by the official TFCP2 FUNCTION field.
Current typed ontology closures establish nucleoplasm→nucleus, cytosol→cytoplasm, GO:0140297→protein binding, and GO:0120513→catalytic complex/protein-containing complex. The nuclear refinement specifies a positive nucleoplasmic pool without claiming that the entire nuclear distribution excludes chromosomes or nucleolus. Broad complex terms are refined to the existing defined synthase complex; no duplicate NEW is proposed.
Other reference scope is deliberately limited. PMID:11013075 is a verified abstract describing the OVCA1/RBM8 interaction screen; publisher access did not yield its full Methods. PMID:8603384 and PMID:8616839 provide historical transcript/candidate-gene context from abstracts, with OVCA2 explicitly separate. PMID:15489334 is an abstract-only MGC resource; PMID:16625196 has a full XML cache but only its sequencing abstract/metadata were used. PMID:25558065 and PMID:29362492 are abstract-only clinical context. PMID:26220823 has a full XML cache, but only its cohort abstract was used here. None supplies invented target biochemical assays or additional developmental-process annotations.
The only newly reproduced source excerpts occur once each in the YAML: seven words from PMID:10519411 and eight from PMID:31463593. Notes and this guide paraphrase rather than repeat those excerpts. Public primary URLs and exact record identifiers remain usable independently of the execution workspace.