Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Identification of potential interaction networks using sequence-based searches for conserved protein-protein interactions or "interologs".
A map of the interactome network of the metazoan C. elegans.
A conserved role of Caenorhabditis elegans CDC-48 in ER-associated protein degradation.
-
Demonstrated CDC-48.1 and CDC-48.2 interact with UFD-1/NPL-4 to form a conserved segregase complex
"In Caenorhabditis elegans two homologs of p97, designated CDC-48.1 and CDC-48.2, exist. Our results indicate that both p97 homologs interact with UFD-1/NPL-4 in a similar CDC-48(UFD-1/NPL-4) complex."
-
RNAi depletion of cdc-48, ufd-1, or npl-4 induces ER stress
"RNAi mediated depletion of the corresponding genes induces ER stress resulting in hypersensitivity to conditions which induce increased levels of unfolded proteins in the ER lumen."
-
The complex is required for ERAD and preventing unfolded protein response activation
"Protein degradation mediated by the ubiquitin/proteasome system is essential for the elimination of misfolded proteins from the endoplasmic reticulum (ER) to adapt to ER stress."
-
UFD-1 depletion causes embryonic lethality
"RNAi mediated depletion of the corresponding genes induces ER stress"
Measuring and modelling mixture toxicity of imidacloprid and thiacloprid on Caenorhabditis elegans and Eisenia fetida.
Cell cycle progression requires the CDC-48UFD-1/NPL-4 complex for efficient DNA replication.
-
CDC-48/UFD-1/NPL-4 complex is required for S phase progression
"Our analysis of the CDC-48(UFD-1/NPL-4) complex identified a general role in S phase progression of mitotic cells essential for embryonic cell division and germline development of adult worms."
-
Depletion causes DNA replication defects and checkpoint activation
"These developmental defects result from activation of the DNA replication checkpoint caused by replication stress."
-
UFD-1 localizes to nucleus and cytoplasm in cell-cycle dependent manner
"Our analysis of the CDC-48(UFD-1/NPL-4) complex identified a general role in S phase progression of mitotic cells"
-
Essential for embryonic cell division and germline development
"essential for embryonic cell division and germline development of adult worms"
Caenorhabditis elegans UBX cofactors for CDC-48/p97 control spermatogenesis.
-
UFD-1 forms a complex with UBXN-3, NPL-4.1, and CDC-48.1
"All six UBXN proteins directly interacted with CDC-48.1 and CDC-48.2"
-
UFD-1 directly interacts with CDC-48.1
"All six UBXN proteins directly interacted with CDC-48.1 and CDC-48.2"
-
ufd-1 RNAi causes germline developmental defects
"simultaneous knockdown of the expression of three genes, ubxn-1, ubxn-2 and ubxn-3, induced embryonic lethal and sterile phenotypes"
Chromatin-associated degradation is defined by UBXN-3/FAF1 to safeguard DNA replication fork progression.
-
CDC-48/UFD-1/NPL-4 complex is required for proper UBXN-3 nuclear localization during S phase
"Indeed, RNAi-mediated depletion of CDC-48 or the cofactor UFD-1/NPL-4 resulted in the formation of UBXN-3-positive punctae in the nucleoplasm of worm embryos"
-
UFD-1/NPL-4 depletion causes UBXN-3 to form punctae in the nucleoplasm
"Indeed, RNAi-mediated depletion of CDC-48 or the cofactor UFD-1/NPL-4 resulted in the formation of UBXN-3-positive punctae in the nucleoplasm of worm embryos"
-
The complex coordinates chromatin-associated degradation of DNA replication factors
"UBXN-3 cooperates with CDC-48 in DNA replication, including degradation of CDT-1 and disassembly of CDC-45/GINS at the chromatin"
-
UFD-1 works with UBXN-3 to promote CDT-1 degradation and GINS disassembly
"UBXN-3 cooperates with CDC-48 in DNA replication, including degradation of CDT-1 and disassembly of CDC-45/GINS at the chromatin"
Deep research report on ufd-1