Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Combined Automated Annotation using Multiple IEA Methods
High-throughput phenotypic characterization of Pseudomonas aeruginosa membrane transport genes.
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Transposon mutants of PA1339, PA1340 and PA1341 are all defective in using L-glutamate as a carbon source; the operon is induced by L-glutamate.
"All three mutants were defective for utilization of L-glutamic acid as a carbon source, and expression of two of these genes was induced by L-glutamic acid"
Characterization of a Pseudomonas putida ABC transporter (AatJMQP) required for acidic amino acid uptake: biochemical properties and regulation by the Aau two-component system.
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The orthologous P. putida KT2440 AatJMQP system takes up Glu and Asp; its binding protein binds Glu and Asp with high affinity and Gln and Asn weakly.
"The purified solute-binding protein AatJ exhibited high affinity towards Glu and Asp (K(d)=0.4 and 1.3 muM, respectively), while Gln and Asn as well as dicarboxylates (succinate and fumarate) were bound with much lower affinity."
UniProt entry Q9I403 (Q9I403_PSEAE)