Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location Vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
STING is an endoplasmic reticulum adaptor that facilitates innate immune signalling.
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Yeast two-hybrid screening identified SSR2/TRAP-beta as a STING1 interacting protein
"we screened an IFN-induced, human fibroblast yeast two-hybrid cDNA library using STING (amino acids 173-379) as a bait and repeatedly isolated Ssr2/TRAPbeta"
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Co-immunoprecipitation confirmed TRAP-beta association with endogenous STING
"we confirmed that TRAPbeta can indeed associate with endogenous STING in HEK 293 cells following co-immunoprecipitation experiments"
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TRAP-beta ablation reduced STING-mediated interferon-beta induction
"loss of TRAPbeta or SEC61beta reduced STING's, ability to induce an IFNbeta promoter driving luciferase"
Visualization of translation and protein biogenesis at the ER membrane.
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Cryo-electron tomography visualization of native ER translocon complexes
"Here we use cryo-electron tomography, extensive classification and molecular modelling to capture snapshots of mRNA translation and protein maturation at the ER membrane"
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Near-complete atomic model of SEC61-TRAP-OSTA complex
"The near-complete atomic model of the most abundant ER translocon variant comprising the protein-conducting channel SEC61, TRAP and the oligosaccharyltransferase complex A (OSTA)"
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TRAP present in both signal peptide and multipass translocon variants
"distinct polysomes bind to different ER translocons specialized in the synthesis of proteins with signal peptides or multipass transmembrane proteins with the translocon-associated protein complex (TRAP) present in both"
Isolation and mapping of the human beta-signal sequence receptor gene (SSR2).
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Original cloning of human SSR2 cDNA
"We have isolated a human cDNA clone homologous to the canine beta-signal sequence receptor gene"
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Identified SSR2 as encoding ER membrane protein involved in translocation
"which codes for an endoplasmic reticulum (ER) membrane protein associated with protein translocation across the ER membrane"
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Mapped SSR2 to chromosome 1q21-q23
"We also localized the human beta-signal sequence receptor gene (SSR2) to chromosome bands 1q21-->q23"
Deep research summary for SSR2/TRAP-beta
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Comprehensive review of TRAP complex structure and function from recent literature
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TRAP is a heterotetramer (alpha, beta, gamma, delta subunits)
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TRAP-beta has type I topology with single transmembrane helix
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TRAP modulates Sec61 lateral gate for weak signal peptides