The review distinguishes the normal publication cache from independently accessible original text. The following locators describe what was inspected; they do not imply inspection of all figures or supplements. Exact current quotations occur only in the review YAML.
| Source | Inspected scope and limits |
|---|---|
| 33229582, original | Selected Methods, Results and Discussion; engineered human GCH1 42–250 and GFRP 1–84. Structural and activity evidence are distinct from the proposed residence-time mechanism. |
| 8068008, author reprint | Indexed original construct descriptions, Results narrative and Discussion, plus authenticated abstract. No independent complete historical sequence alignment, figure pixels or table-rate columns. |
| 11087827 | Selected original Methods and Results after direct PMC recovery. Human engineered deletion and bacterial mutagenesis have separate scopes. |
| 16696853, original | Selected original Methods/Results; human GCH1, human AHSA1 and rat GFRP retain separate scopes. |
| 21988832 | Original corrected Table S2 row 782 and Methods identify GCH1–YWHAZ. The author-name correction does not alter that row; clone sequence and physiological consequence remain distinct questions. |
| 32296183 | Six retained original PSI records cover the two transportin partners. Yeast screen/product labels are retained; no individual orthogonal-assay or import mechanism claim. |
| 15604419, indexed original | Original selected endothelial Methods/Results include a stated negative LPS result. Publisher access was restricted and direct mirror retrieval returned HTML; the readable indexed original, not that failed file, supports the interpretation. |
| 19666465 | Indexed original selected Methods/Results/Discussion distinguish human GCH1 transgene in murine NIH3T3 cells from endogenous murine endothelial knockdown. The methotrexate antioxidant-capacity experiment is a separate intervention. |
| 17717598, original | Selected original Results, Discussion and Methods: human associations and a UTR reporter do not directly measure NOS catalytic activity or natural GCH1 protein isoforms. |
| 25557619 | Selected original Doppler, treatment and developmental Results/Discussion/Figure 4 text. Global-null mouse bradycardia and partial survival rescue do not establish a demonstrated heart-rate rescue. |
| 17057711 | Authentic abstract and publisher abstract only; rat pain interventions and separate human haplotypes are distinguished. Full experimental controls were not independently read. |
| 11087823, original | Complete original main text concerns PAX6 cis-regulation, not GCH1 complex activity. This supports MISCITED; nearby PMID 11087827 is not guessed to be the intended identifier. |
The Human Protein Atlas GCH1 page was inspected as target-specific text/table evidence: nucleoplasm in A-431, U-251MG and U2OS, cytosol in U-251MG/U2OS, and nuclear membrane in U2OS. Antibody images were not independently interpreted.
The official Reactome graph was checked through every relevant component edge. R-HSA-1474146 uses catalyst 1474128, human decamer 1474144, dimer 1474143 and GCH1 entity 1474127, resolving to P30793 residues 1–250 in cytosol. R-HSA-1474158 contains the same target in input/output complexes. Its inhibitory catalyst 9959889 instead resolves through 1474155/1474159 to P30047 GCHFR. These are model-supported locations, not newly inspected microscopy experiments.
The exact PANTHER19.0 source URLs, target path and sequence hashes are in the evidence inventory. The unqualified inherited review is preserved unchanged through the historical archive; its absent qualifiers remain unknown.
PMID:7678411, indexed original Methods/Results describe HUVEC passages 3–8, 24-hour enzyme/pterin measurements and increased activity after LPS. Indexed dose typography is ambiguous; no figure pixels were inspected. The separate PMID:15604419 Results report absent protein induction, with data not shown. Endpoint differences do not explain the discrepancy. UniProt's collective induction bibliography does not override either experiment.
GO:0034615 GCH1 complex represents the catalytic assembly, with verified is_a paths to protein-containing complex through catalytic complex and intracellular protein-containing complex. Human homodecamer structures support this refinement; GFRP binding does not require inventing another complex term.
PMID:17704208(https://pubmed.ncbi.nlm.nih.gov/17704208/) is available here as an authentic abstract: it distinguishes laminar from oscillatory shear and CK2 alpha-prime from the separate astrocyte alpha-subunit pair. Its phosphorylation endpoint is not an additional direct interaction assay for P68400. Full Methods were not inspected.