Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Integrin-mediated cell adhesion: the cytoskeletal connection.
Recruitment of the Arp2/3 complex to vinculin: coupling membrane protrusion to matrix adhesion.
Structural basis for amplifying vinculin activation by talin.
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Talin VBSs bind the vinculin D1 head and displace the tail (activation by helical bundle conversion).
"all of talin VBSs activate vinculin by provoking helical bundle conversion of the Vh domain, which displaces the vinculin tail (Vt) domain"
RNA and RNA binding proteins participate in early stages of cell spreading through spreading initiation centers.
Cytoskeletal proteins talin and vinculin in integrin-mediated adhesion.
Vinculin--a dynamic regulator of cell adhesion.
Structural dynamics of alpha-actinin-vinculin interactions.
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alpha-actinin binds vinculin D1 via an alpha-helical VBS.
"alpha-Actinin interacts with vinculin through the binding of an alpha-helix (alphaVBS) present within the R4 spectrin repeat of its central rod domain to vinculin's N-terminal seven-helical bundle domain (Vh1)"
Proteomics of human umbilical vein endothelial cells applied to etoposide-induced apoptosis.
Towards a proteome-scale map of the human protein-protein interaction network.
Role of dystrophins and utrophins in platelet adhesion process.
CAP interacts with cytoskeletal proteins and regulates adhesion-mediated ERK activation and motility.
Vinculin binding in its closed conformation by a helix addition mechanism.
Human alpha-synemin interacts directly with vinculin and metavinculin.
beta-Dystroglycan modulates the interplay between actin and microtubules in human-adhered platelets.
Large-scale proteomics and phosphoproteomics of urinary exosomes.
Proteomic analysis of increased Parkin expression and its interactants provides evidence for a role in modulation of mitochondrial function.
Vinculin regulates cell-surface E-cadherin expression by binding to beta-catenin.
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Vinculin binding to beta-catenin stabilizes surface E-cadherin and cell-cell adhesion.
"the reduced surface E-cadherin expression could be rescued by introduction of vinculin, but not of a vinculin A50I substitution mutant that is defective for β-catenin binding"
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Complete sequence of human vinculin and assignment of the gene to chromosome 10.
Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation.
Novel vinculin binding site of the IpaA invasin of Shigella.
The rickettsia surface cell antigen 4 applies mimicry to bind to and activate vinculin.
KIAA1797/FOCAD encodes a novel focal adhesion protein with tumour suppressor function in gliomas.
Platelet proteome analysis reveals integrin-dependent aggregation defects in patients with myelodysplastic syndromes.
Proteomic analysis of cerebrospinal fluid extracellular vesicles: a comprehensive dataset.
A proteome-scale map of the human interactome network.
E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
ZO-1 controls endothelial adherens junctions, cell-cell tension, angiogenesis, and barrier formation.
Dual role of vinculin in barrier-disruptive and barrier-enhancing endothelial cell responses.
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Vinculin partitions between talin (FA) and VE-cadherin (AJ) depending on agonist and tension.
"Thrombin stimulated the vinculin association with FA protein talin and suppressed the interaction with AJ protein, VE-cadherin."
The WD40-domain containing protein CORO2B is specifically enriched in glomerular podocytes and regulates the ventral actin cytoskeleton.
An interactome perturbation framework prioritizes damaging missense mutations for developmental disorders.
Blood-Brain Barrier: From Physiology to Disease and Back.
F-actin binding site masked by the intramolecular association of vinculin head and tail domains.
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Full-length vinculin is autoinhibited for F-actin binding; the isolated tail binds F-actin.
"Cosedimentation and crosslinking assays, and direct visualization by transmission electron microscopy, reveal an interaction between F-actin and a bacterially expressed fusion protein containing amino acids 811-1066 of vinculin"
Monocyte cells and cancer cells express novel paxillin isoforms with different binding properties to focal adhesion proteins.
Spatial and temporal expression of the beta1D integrin during mouse development.
alpha-Catenin-vinculin interaction functions to organize the apical junctional complex in epithelial cells.
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alphaE-catenin binds vinculin; vinculin-null F9 cells have impaired apical junction organization rescued by vinculin.
"In vinculin-null F9 cells, their apical junctional organization was impaired, and this phenotype was rescued by reexpression of vinculin."
Vinexin: a novel vinculin-binding protein with multiple SH3 domains enhances actin cytoskeletal organization.
Release Of ADP From Myosin
Release of platelet cytosolic components
Exocytosis of secretory granule lumen proteins
Exocytosis of specific granule lumen proteins
Exocytosis of ficolin-rich granule lumen proteins
Calpain2 (m-Calpain, CAPN2:CAPNS:Ca2+) cleaves VCL (Vinculin)
CDH1-associated CTNNA1 binds VCL
CDH1 forms homotypic trans-dimers
Analysis of a vinculin homolog in a sponge (phylum Porifera) reveals that vertebrate-like cell adhesions emerged early in animal evolution.
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Sponge vinculin is activated by talin for F-actin binding, as in vertebrates.
"Thus, binding of talin to Op vinculin D1 releases autoinhibition for F-actin binding, similar to the behavior of vertebrate vinculin."
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Sponge vinculin binds talin but not a beta-catenin peptide.
"Moreover, Op vinculin bound talin but not β-catenin"
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Vinculin/alpha-catenin family proteins are present in close outgroups of animals.
"VIN proteins link actin filaments to membrane proteins at the plasma membrane and are found in all animals and their close outgroups (choanoflagellates, chytridomycetes, apusozoa, amoebozoa)"
Integrin-Mediated Adhesion in the Unicellular Holozoan Capsaspora owczarzaki.
Ancient origin of the integrin-mediated adhesion and signaling machinery.
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Talin, vinculin and paxillin scaffolds originated before animals.
"Many of the scaffolding proteins (talin, vinculin, paxillin) most likely evolved in the common ancestor of amoebozoans and opisthokonts, where they had ancestrally different functions (as in present day amoebozoans)."