parE (PA4967, Q9HUJ8) notes

Literature

No deep-research provider was available. PubMed E-utilities returned HTTP 500
on 2026-11-05. The review rests on the full text of PMID:10049263 (cached) and
HAMAP MF_00938.

ParC and ParE purified separately as MBP fusions PMID:10049263.
Reconstituted enzyme activities PMID:10049263.
A secondary fluoroquinolone target, less sensitive than gyrase.

PseudoCAP IBA-seed assessment

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN000018885	GO:0009330 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex	3	117 (78 taxa)	GO:0009340 DNA topoisomerase IV complex, IDA, PMID:10049263 (PseudoCAP)	SOUND	Full text read. Activity reconstituted from purified ParC + ParE; the seed term is a descendant of the node term, which is valid IBD evidence.	E. coli GyrB P0AES6, Aquifex GyrB O67137	Yes. The generic type II complex term suits a GyrB/ParE node, and E. coli gyrase is a well-characterised complex.
PTN000018885	GO:0006265 DNA topological change	2	117 (78 taxa)	GO:0006265, IDA, PMID:10049263 (PseudoCAP)	SOUND	Decatenation and relaxation are catalysed by the reconstituted enzyme.	E. coli GyrB P0AES6	Yes. E. coli GyrB alone grounds it.
PTN000018885	GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	5	116 (77 taxa)	GO:0003918, IDA, PMID:10049263 (PseudoCAP)	SOUND	ATP-dependent decatenation and relaxation by the reconstituted enzyme meets the definition.	E. coli GyrB P0AES6, M. tuberculosis GyrB P9WG45, Arabidopsis AT5G04130, P. falciparum Top2 Q8I528	Yes. Several directly assayed seeds.

No PSEUDOCAP and no GO_CENTRAL defect.

Other decisions

GO:0009330 IBA: TOO_GENERAL, because GO:0009340 is accepted.
GO:0007059 chromosome segregation (IEA): ACCEPT; decatenation is the
segregation step the enzyme performs.
GO:0051276, GO:0003677, GO:0005524, GO:0005694: KEEP_AS_NON_CORE.