Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
Different effects of Sec61α, Sec62 and Sec63 depletion on transport of polypeptides into the endoplasmic reticulum of mammalian cells.
Chaperone-Mediated Sec61 Channel Gating during ER Import of Small Precursor Proteins Overcomes Sec61 Inhibitor-Reinforced Energy Barrier.
Intrinsically Disordered Protein TEX264 Mediates ER-phagy.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Large-scale phosphomimetic screening identifies phospho-modulated motif-based protein interactions.
Targeting cell migration and the endoplasmic reticulum stress response with calmodulin antagonists: a clinically tested small molecule phenocopy of SEC62 gene silencing in human tumor cells.
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SEC62 regulates the major ER Ca2+ leak channel Sec61 via a direct, Ca2+-sensitive interaction (Biacore); a Ca2+-binding motif in SEC62 is essential for this function. SEC62 silencing elevates cytosolic Ca2+ and increases thapsigargin-evoked ER Ca2+ leakage, and calmodulin antagonists (trifluoperazine, ophiobolin A) phenocopy SEC62 depletion (impaired migration, ER-stress sensitization). SEC62 is overexpressed in prostate, lung and thyroid cancers and correlates with reduced survival.
Identification of signal peptide features for substrate specificity in human Sec62/Sec63-dependent ER protein import.
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Using an unbiased proteomics approach in intact human cells, 22 novel Sec62/Sec63 substrates were identified in addition to ERj3; these substrates share signal peptides with comparatively longer but less hydrophobic H-regions and lower C-region polarity, and the combination of a slowly gating signal peptide plus a downstream translocation-disruptive positively charged cluster is decisive for the Sec62/Sec63 (and BiP) requirement. The human Sec62/Sec63 complex may support Sec61 opening either by direct interaction with the cytosolic N-terminus of Sec61alpha or via recruitment of BiP to ER-lumenal loop 7 of Sec61alpha.
Rules of Engagement for Components of Membrane Protein Biogenesis at the Human Endoplasmic Reticulum.
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Review/article hybrid using siRNA depletion of individual ER targeting and insertion components (including SEC62/SEC63) combined with label-free quantitative proteomics to define client types and rules of engagement for components of the human ER protein biogenesis machinery, placing SEC62 as a substrate-selective Sec61 translocon effector.
Identification of a human cDNA homologue to the Drosophila translocation protein 1 (Dtrp1).
UniProt entry Q99442 (SEC62_HUMAN), Translocation protein SEC62
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SEC62 mediates post-translational transport of precursor polypeptides across the ER, acts as a targeting receptor for small presecretory proteins, positions them into the Sec61 channel and triggers channel opening; multi-pass ER membrane protein; auxiliary component of the Sec61 translocon with SEC63; interacts with GABARAP (ER-phagy).