Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
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Matsuyama et al. reported a proteome-scale S. pombe ORFeome/YFP localization survey covering 4,431 proteins.
"Next, we determined the localization of 4,431 proteins, corresponding to approximately 90% of the fission yeast proteome, by tagging each ORF with the yellow fluorescent protein."
UniProt entry Q9UT88 (ERG8_SCHPO)