Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions.
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Direct AREB1/AREB2 promoter binding and ABRE reporter activation establish ABA-dependent transcription in vegetative drought and salt response.
"both the AREB1 and AREB2 proteins activated transcription of a reporter gene driven by ABRE."
bZIP transcription factors in Arabidopsis.
ABF2, an ABRE-binding bZIP factor, is an essential component of glucose signaling and its overexpression affects multiple stress tolerance.
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ABF2 single-mutant glucose defects are distinct from ABF3/4 stress phenotypes; overexpression effects do not establish obligatory single-gene drought function.
"ABF2 overexpression promoted glucose-induced inhibition of seedling development, whereas its mutation impaired glucose response."
ARIA, an Arabidopsis arm repeat protein interacting with a transcriptional regulator of abscisic acid-responsive gene expression, is a novel abscisic acid signaling component.
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ARIA interaction modulates ABF2; fluorescent localization directly places ABF2 in the nucleus.
"ABF2 is a transcription factor and, as shown in Figure 2C (top section), is localized in the nucleus."
AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis.
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Activated AREB1DeltaQT and dominant-repressor constructs establish transcriptional control of vegetative drought responses; intact overexpression alone is insufficient without activation.
"expression of the intact AREB1 gene on its own is insufficient to lead to expression of downstream genes under normal growth conditions."
Arabidopsis calcium-dependent protein kinase AtCPK32 interacts with ABF4, a transcriptional regulator of abscisic acid-responsive gene expression, and modulates its activity.
Transcriptional regulation of ABI3- and ABA-responsive genes including RD29B and RD29A in seeds, germinating embryos, and seedlings of Arabidopsis.
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Direct comparison shows AREB1 and ABI5 activate RD29B more strongly than RD29A.
"ABI3, ABI5 and AREB1 activated transcription of a GUS reporter gene driven by the RD29B promoter strongly"
Arabidopsis NF-YB subunits LEC1 and LEC1-LIKE activate transcription by interacting with seed-specific ABRE-binding factors.
In vitro reconstitution of an abscisic acid signalling pathway.
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Direct PYR1-ABI1-OST1-ABF2 reconstitution connects ABA perception to ABF2 phosphorylation and gene expression. ABF2 is a kinase substrate, not the kinase.
"Introduction of these four components into plant protoplasts results in ABA-responsive gene expression."
An Arabidopsis gene regulatory network for secondary cell wall synthesis.
ABF2, ABF3, and ABF4 Promote ABA-Mediated Chlorophyll Degradation and Leaf Senescence by Transcriptional Activation of Chlorophyll Catabolic Genes and Senescence-Associated Genes in Arabidopsis.
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ABF2/3/4 directly bind and activate chlorophyll-catabolic promoters; stay-green genetics uses the triple mutant and cannot isolate ABF2 necessity.
"ABF2, ABF3, and ABF4 directly bound to and activated the NYE1 promoter in vitro and in vivo."
Trehalose-6-phosphate phosphatase E modulates ABA-controlled root growth and stomatal movement in Arabidopsis.
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ABF2 binds the TPPE promoter, demonstrated by Y1H, EMSA, ChIP and reporter activation. TPPE is a transcriptional target, not a demonstrated ABF2 protein-binding partner.
"directly binds to the TPPE promoter to activate its expression."
Abscisic acid-dependent multisite phosphorylation regulates the activity of a transcription activator AREB1.
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Multisite phosphorylation controls AREB1 transactivation; phosphomimic mutants activate targets without exogenous ABA.
"the ABA-dependent multisite phosphorylation of AREB1 regulates its own activation in plants."