Phospholipid Remodeling and Cholesterol Availability Regulate Intestinal Stemness and Tumorigenesis
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Identification and characterization of a major liver lysophosphatidylcholine acyltransferase.
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Identified LPCAT3 as the major hepatic lysophosphatidylcholine acyltransferase. LPCAT3 belongs to the MBOAT family, is ER-localized, and shows substrate preference for unsaturated fatty acids. Knockdown in Huh7 cells virtually eliminates membrane LPCAT activity.
"we report here the identification of a novel LPCAT, which we named LPCAT3. LPCAT3 belongs to the membrane-bound O-acyltransferase (MBOAT) family... In a human hepatoma Huh7 cells, RNA interference-mediated knockdown of LPCAT3 resulted in virtually complete loss of membrane LPCAT activity"
Lysophospholipid acyltransferases and arachidonate recycling in human neutrophils.
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Characterized substrate specificity of MBOAT5/LPCAT3 using MS-based assays. MBOAT5 prefers LPC and lyso-PS with linoleoyl and arachidonoyl acyl-CoA donors. Activity is thimerosal-sensitive. Implicates MBOAT5 in arachidonate recycling and regulation of free AA for leukotriene synthesis.
"MBOAT5 prefers lysophosphatidylcholine and lyso-PS to incorporate linoleoyl and arachidonoyl chains. MBOAT7 is a lysophosphatidylinositol acyltransferase with remarkable specificity for arachidonoyl-CoA. MBOAT5 and MBOAT7 are particularly susceptible to inhibition by thimerosal."
Member of the membrane-bound O-acyltransferase (MBOAT) family encodes a lysophospholipid acyltransferase with broad substrate specificity.
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Demonstrated that MBOAT5/LPCAT3 is a lysophospholipid acyltransferase acting on LPC, LPS, and LPE. Knockdown reduced PUFA incorporation into PC, PS, and PE. Overexpression increased LPC, LPS, and LPE acyltransferase activities but not LPIAT or LPAAT activities.
"These results indicate that human MBOAT5 is a lysophospholipid acyltransferase acting preferentially on LPC, LPS and LPE."
Defining the membrane proteome of NK cells.
Lysophosphatidylcholine acyltransferase 3 knockdown-mediated liver lysophosphatidylcholine accumulation promotes very low density lipoprotein production by enhancing microsomal triglyceride transfer protein expression.
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Demonstrated that LPCAT3 is the major hepatic isoform. Knockdown increases LysoPC, decreases certain PC species, and reduces hepatic triglycerides. Paradoxically, knockdown increases plasma TG and apoB through enhanced VLDL secretion via increased MTP expression.
"we found that LPCAT3 is the major hepatic isoform, and its knockdown significantly reduces hepatic LPCAT activity... these results indicate that hepatic LPCAT3 modulates VLDL production by regulating LysoPC levels and MTP expression"
2-acyl LPC is acylated to PC by LPCAT
1-acyl LPC is acylated to PC by LPCAT
1-acyl LPS is acylated to PS by LPSAT
2-acyl LPE is acylated to PE by LPEAT
1-acyl LPE is acylated to PE by LPEAT
2-acyl LPS is acylated to PS by LPSAT
Acyl chain remodelling of PC
Acyl chain remodelling of PS
Acyl chain remodelling of PE
Deep research report on LPCAT3