Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Mutational analysis of the OprM outer membrane component of the MexA-MexB-OprM multidrug efflux system of Pseudomonas aeruginosa.
Structure of the periplasmic component of a bacterial drug efflux pump.
In vitro transport activity of the fully assembled MexAB-OprM efflux pump from Pseudomonas aeruginosa.
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Reconstituted MexAB-OprM performs energy-dependent transport.
"By combining proteoliposomes containing the MexAB and OprM portions of the complex, we are able to assay energy-dependent substrate translocation in a system that mimics the dual-membrane architecture of Gram-negative bacteria."
Structures of the wild-type MexAB-OprM tripartite pump reveal its complex formation and drug efflux mechanism.
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A MexA hexamer joins MexB and OprM.
"MexB does not directly contact OprM; instead, MexA joins MexB and OprM by forming a funnel-like hexamer."
Antibiotic export by MexB multidrug efflux transporter is allosterically controlled by a MexA-OprM chaperone-like complex.
Role of mexA-mexB-oprM in antibiotic efflux in Pseudomonas aeruginosa.
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mexA null mutants accumulate more antibiotic and are more susceptible.
"Thus, the steady-state accumulation levels of tetracycline and norfloxacin were increased in mexA and oprM null mutants."
Active efflux and diffusion are involved in transport of Pseudomonas aeruginosa cell-to-cell signals.
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MexAB-OprM effluxes 3OC12-HSL.
"Instead, the mexA-mexB-oprM-encoded efflux pump is involved in active efflux of 3OC12-HSL."