CDC21 (TMP1; YOR074C, P06785) notes

• Thymidylate synthase, EC 2.1.1.45: dUMP + CH2-THF -> dTMP + DHF [UniProt:P06785].
• tmp1 and cdc21 mutants lack the activity: "Extracts of a strain bearing the temperature-sensitive cell division cycle mutation cdc21 lacked detectable thymidylate synthetase activity under all conditions tested." PMID:334734
• cdc21 and tmp1 are the same gene and the mutant is a dTMP auxotroph PMID:794696.
• The cloned TMP1 restores activity and complements E. coli thyA PMID:6287238.
• Location: "immunofluorescence analysis suggests that thymidylate synthase is situated at the nuclear periphery" PMID:8132557.
• The gene is required for nuclear and mitochondrial DNA synthesis [UniProt:P06785].

Curation decisions

• The mitochondrion IBA (from human TYMS and plant DHFR-TS) and the cytosol IBA (from E. coli) are marked over-annotated. Yeast data place the protein at the nuclear periphery.
• The cytosol RCA rows are marked over-annotated for the same reason.
• GO:0006207 (de novo pyrimidine nucleobase biosynthesis) is changed to GO:0006231 (dTMP biosynthesis).
• The folate cycle and folic acid metabolic rows are marked over-annotated.
• The chorismate row is removed.