Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Genetic interactions due to constitutive and inducible gene regulation mediated by the unfolded protein response in C. elegans.
Caenorhabditis elegans drp-1 and fis-2 regulate distinct cell-death execution pathways downstream of ced-3 and independent of ced-9.
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fis-2 (a homolog of human Fis1) has a minor pro-apoptotic role revealed in sensitized genetic backgrounds, acting downstream of the CED-3 caspase and independently of DRP-1 and CED-9 to promote elimination of mitochondria in dying cells.
"minor proapoptotic roles for drp-1 and fis-2, a homolog of human Fis1, are revealed in sensitized genetic backgrounds"
Mutations in Fis1 disrupt orderly disposal of defective mitochondria.
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C. elegans fis-2 single and fis-1;fis-2 double mutants have wild-type mitochondrial (and peroxisomal) morphology; Fis1 homologs are not essential for fission or for DRP-1 recruitment, unlike MFF/DRP-1.
"Our results show that fis-1 and fis-2 single and double mutants have wild-type mitochondrial morphologies, as also shown by others"
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Loss of both FIS1 paralogs (the fis-1;fis-2 "Fis1" double mutant) causes stress-induced accumulation of large LGG-1 autophagic aggregates containing mitochondrial remnants and DRP-1; drp-1 fis-1 fis-2 and pink-1 fis-1 fis-2 triple mutants place fis-2 genetically in the mitophagic disposal pathway downstream of DRP-1/MFF and PINK-1.
"pink-1 single and pink-1 fis-1 fis-2 triple mutant animals had no colocalizing spots, as expected for a complete block of mitophagy"