Annotation inferences using phylogenetic trees
Gene Ontology annotation through association of InterPro records with GO terms
Combined Automated Annotation using Multiple IEA Methods
A reference map of the human binary protein interactome.
Human UFSP1 translated from an upstream near-cognate initiation codon functions as an active UFM1-specific protease.
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Human UFSP1 is translated from an upstream near-cognate 217CUG codon (eIF2A-mediated), yielding a form that contains a catalytic protease domain with a Cys active site; the canonical 445AUG form lacks the active site.
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Both UFSP1 and UFSP2 mediate maturation of the UFM1 precursor and de-UFMylation of target proteins; mutation of the catalytic cysteine abolishes activity.
Human UFSP1 is an active protease that regulates UFM1 maturation and UFMylation.
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UFSP1 (from a non-canonical start site) is an active protease; cells lacking both UFSPs show complete loss of UFMylation from absence of mature UFM1.
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UFSP1 acts early to mature UFM1 and cleave a potential autoinhibitory UFM1 modification on UFC1; unlike UFSP2 it does not remove UFM1 from RPL26. UFSP1 is cytosolic and disassembles polyUFM1 chains in vitro.