Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Disruptions of the Arabidopsis Enoyl-CoA reductase gene reveal an essential role for very-long-chain fatty acid synthesis in cell expansion during plant morphogenesis.
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cer10 mutants disrupt ECR; loss of ECR reduces wax and alters seed TAG and sphingolipid VLCFAs; functional GFP-ECR is on the ER membrane; developmental defects linked to sphingolipids and cell expansion.
"demonstrating in planta that ECR is involved in all VLCFA elongation reactions in Arabidopsis"
Mapping the Arabidopsis organelle proteome.
Chrysotile asbestos and health in Zimbabwe: I. Analysis of miners and millers compensated for asbestos-related diseases since independence (1980).
Putative glycosyltransferases and other plant Golgi apparatus proteins are revealed by LOPIT proteomics.
Arabidopsis GLASSY HAIR genes promote trichome papillae development.
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glh6 is a new cer10 allele; glh6 trichomes show papillae, branching and cuticle defects, possibly indirect consequences of developmental arrest.
"the glh6 mutants showed defects in leaf cuticular wax, and glh6 was found to represent a new allele of the eceriferum 10 (cer10) mutation"
Global Analysis of Membrane-associated Protein Oligomerization Using Protein Correlation Profiling.
Arabidopsis Bax inhibitor-1 interacts with enzymes related to very-long-chain fatty acid synthesis.
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AtBI-1 co-purifies with AtELO2, KCR1, PAS2, CER10 and AtCb5-D.
"AtBI-1 forms a complex with AtELO2, KCR1, PAS2, CER10, and AtCb5-D"
Functional characterization of the Arabidopsis thaliana orthologue of Tsc13p, the enoyl reductase of the yeast microsomal fatty acid elongating system.
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At3g55360 complements yeast tsc13 enoyl reductase deficiency, interacts with Elo2p/Elo3p, and appears to be the sole microsomal enoyl reductase in Arabidopsis.
"heterologous expression of At3g55360 functionally complements the temperature-sensitive phenotype of a yeast tsc13 mutant that is deficient in enoyl reductase activity"
The very-long-chain hydroxy fatty acyl-CoA dehydratase PASTICCINO2 is essential and limiting for plant development.
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BiFC shows PAS2 interacts with ECR/CER10 in the ER.
"PAS2 was specifically associated in the endoplasmic reticulum with the enoyl-CoA reductase CER10, the fourth enzyme of the elongase complex"
Falcon deep research report for ECR (At3g55360)
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Synthesizes ECR/CER10 as the ER membrane very-long-chain enoyl-CoA reductase catalyzing the final step of each elongation cycle, with broad chain-length specificity.
"This localization was demonstrated through GFP fusion studies showing that functional GFP-ECR labels a reticulate network characteristic of the ER"