Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
The fourth arginine catabolic pathway of Pseudomonas aeruginosa.
-
Defines the D-arginine dehydrogenase route (2-ketoarginine to 4-guanidinobutyrate to 4-aminobutyrate) with an inducible guanidinobutyrase, into which L-arginine is partly channelled when the AST pathway is blocked.
"2-ketoarginine was catabolized further to 4-guanidinobutyraldehyde, 4-guanidinobutyrate and 4-aminobutyrate."
Characterization and regulation of the gbuA gene, encoding guanidinobutyrase in the arginine dehydrogenase pathway of Pseudomonas aeruginosa PAO1.
-
Purified GbuA is specific for 4-guanidinobutyrate, Mn2+-dependent, and required for growth on 4-guanidinobutyrate; it has no activity on 3-guanidinopropionate.
"No enzyme activity was detected with other guanidino compounds, including arginine, agmatine, 3-guanidinopropionate, and guanidinoacetate."
Crystal structures of Pseudomonas aeruginosa guanidinobutyrase and guanidinopropionase, members of the ureohydrolase superfamily.
Functional genomics enables identification of genes of the arginine transaminase pathway in Pseudomonas aeruginosa.
-
gbuA is L-arginine inducible independently of ArgR, and its disruption abolishes growth on L-arginine in an AST-deficient (aruF) background.
"disruption of gbuA abolished growth on l -arginine"
Guanidino acid hydrolysis by the human enzyme annotated as agmatinase.