Gene Ontology annotation through association of InterPro records with GO terms
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Combined Automated Annotation using Multiple IEA Methods
UniProtKB record A0A0H2MNF8 (A0A0H2MNF8_VARPD) - Taurine import ATP-binding protein TauB from Variovorax paradoxus TBEA6
-
VPARA_03660 (tauB1, A0A0H2MNF8) is a 262-aa peripheral inner-membrane ABC transporter ATP-binding subunit (PROSITE PS50893, residues 6-235; PANTHER PTHR42788:SF18) carrying a conserved Walker A ATP-binding motif at residues 40-47 (40-GASGCGKT-47).
"FT BINDING 40..47
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00434""
-
VPARA_03660 (tauB1) corresponds to EMBL CDS accession KLN58255.1 on genomic contig JZWI01000003, where consecutive CDS accessions (KLN58254.1-KLN58256.1) and locus tags stepping by 10 place tauB1 immediately between tauA (VPARA_03650, KLN58254.1) and tauC (VPARA_03670, KLN58256.1).
"DR EMBL; JZWI01000003; KLN58255.1; -; Genomic_DNA."
Identification of sulfate starvation-regulated genes in Escherichia coli: a gene cluster involved in the utilization of taurine as a sulfur source.
-
Characterization of the E. coli tauABCD operon identified TauB as the ATP-binding subunit of the taurine ABC importer.
"The TauA protein contained a putative signal peptide for transport into the periplasm; the TauB and TauC proteins showed sequence similarity to ATP-binding proteins and membrane proteins, respectively, of ABC-type transport systems"
Deletion analysis of the Escherichia coli taurine and alkanesulfonate transport systems.
-
Deletion and complementation analysis in E. coli established that TauABC mediates taurine import and its subunits cannot form functional hybrid transporters with SsuABC.
"Mutants in which only formation of hybrid transporters was possible were unable to grow with sulfonates, indicating that the individual components of the two transport systems were not functionally exchangeable."