Gene Ontology annotation through association of InterPro records with GO terms.
Annotation inferences using phylogenetic trees
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara.
Electronic Gene Ontology annotations created by ARBA machine learning models
Molecular cloning of testican-2: defining a novel calcium-binding proteoglycan family expressed in brain.
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Original characterization paper defining testican-2 as a calcium-binding proteoglycan with modular domain structure
"It encodes a protein precursor of 424 amino acids that consists of a signal peptide, a follistatin-like domain, a Ca2+-binding domain, a thyroglobulin-like domain, and a C-terminal region with two putative glycosaminoglycan attachment sites."
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Calcium binding was experimentally demonstrated using circular dichroism spectroscopy
"A recombinant fragment consisting of the Ca2+-binding EF-hand domain and the thyroglobulin-like domain of testican-2 showed a reversible Ca2+-dependent conformational change in circular dichroism studies."
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Highly expressed in CNS with widespread neuronal expression
"A widespread expression in multiple neuronal cell types in olfactory bulb, cerebral cortex, thalamus, hippocampus, cerebellum, and medulla was detected by in situ hybridization."
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Proposes potential role in neurogenesis
"Testican-1 and -2 form a novel Ca2+-binding proteoglycan family built of modular domains with the potential to participate in diverse steps of neurogenesis."
Testican 2 abrogates inhibition of membrane-type matrix metalloproteinases by other testican family proteins.
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Testican-2 uniquely lacks the MT-MMP inhibitory activity of other testican family members
"The inhibitory function is common among testican family members except for testican 2, which was shown to uniquely abolish inhibition of MT1-MMP- or MT3-MMP-mediated pro-MMP-2 activation by other testican family members."
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Testican-2 inactivates other testican proteins through direct binding
"Testican 2 inactivates N-Tes by binding to the COOH-terminal extracellular calcium-binding domain of N-Tes through its NH(2)-terminal unique domain as demonstrated by coimmunoprecipitation analysis"
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Testican-2 promotes cell motility by abrogating testican inhibition of MT-MMPs
"Migration of U251 cells on collagen, which was dependent on MT1-MMP activity under serum-free condition, was inhibited by N-Tes or N-Tes-Delta 122 deposited on collagen."
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Testican-2 binds to other testicans deposited on ECM components
"Testican 2 was not incorporated into collagen by itself, and was deposited only in the presence of N-Tes, suggesting that testican 2 binds to N-Tes deposited on collagen."
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All testican family members are expressed in normal brain neurons
"In situ hybridization showed that neurons are a major source of all of the testican family members in the normal brain."
A reference map of the human binary protein interactome.
Deep research report on SPOCK2