file:PSEPK/PP_3206/PP_3206-uniprot.txt

UniProtKB entry Q88HZ6 for Pseudomonas putida KT2440 PP_3206

The unreviewed target record identifies an NAD-dependent epimerase/dehydratase-fold protein but supplies no substrate or pathway annotation.

"InterPro; IPR001509; Epimerase_deHydtase."

Rhizobia catabolize nod gene-inducing flavonoids via C-ring fission mechanisms.

Pseudomonas putida was experimentally observed to degrade quercetin through A-ring fission.

"Pseudomonas putida degraded quercetin via an initial fission in its A-ring"

Genetic and functional characterization of a novel meta-pathway for degradation of naringenin in Herbaspirillum seropedicae SmR1.

The Herbaspirillum naringenin pathway generates oxaloacetate after A-ring meta-cleavage, but FdeJ itself was not among the genes directly tested.

"After meta-cleavage of the A-ring, the subsequent metabolic steps generate oxaloacetic acid that is metabolized via the tricarboxylic acid cycle."

file:PSEPK/PP_3206/PP_3206-deep-research-openscientist.md

OpenScientist deep research report on PP_3206 (Q88HZ6)

The report identifies the K26183/R13076 assignment and explicitly notes that FdeJ lacks direct biochemical validation.

"FdeJ's specific reaction (oxaloacetate-releasing hydrolysis, KEGG R13076) is therefore a pathway/orthology inference"

file:PSEPK/PP_3206/PP_3206-bioinformatics/RESULTS.md

Reproducible PP_3206 and Hsero_1012 sequence comparison

Q88HZ6 and D8J0X4 share 51.1% identity over 311 aligned residue pairs.

"Pairwise identity over aligned residue pairs: 51.1%"