Gene Ontology annotation through association of InterPro records with GO terms
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
UniProt record for hippo (F2UQC7)
A fast and robust gene knockout method for <i>Salpingoeca rosetta</i> informs the genetics of choanoflagellate multicellular development
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hippo knockout cells proliferate more slowly than wild type.
"On the other hand, hippopac1 and warts pac1 KO clones proliferated markedly slower"
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hippo knockout rosettes are of normal size.
"The size of hippopac1 and yorkiepac1 rosettes did not significantly differ from wild type (11.6 ± 1.7 cells and 9.8 ± 3.1 cells"
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All knockout clones, including hippo, still develop into rosettes.
"all six KO clones reliably developed into rosettes, as did wild-type cells"
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The slower proliferation of hippo and warts knockouts is opposite to the animal loss-of-function phenotype.
"down proliferation in S. rosetta, which is the opposite of their animal loss-of-function phenotype"
A selection-based knockout approach for a choanoflagellate reveals regulation of multicellular development by Hippo signaling.
Premetazoan origin of the hippo signaling pathway.
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Hpo homologs are defined by a Ste20-like kinase domain plus a SARAH domain.
"Our searches further identified homologues of Hpo, defined by the presence of a Ste20-like kinase domain and a SARAH domain"
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The Capsaspora Hippo kinase can activate the Drosophila Hippo cascade.
"Interestingly, Co-Hpo also stimulated the phosphorylation of Dm-Wts and Dm-Yki, as revealed by phospho-specific antibodies against P-Dm-Wts-T1077 and P-Dm-Yki-S168, respectively (Figure 4D)."
The Hippo kinase cascade regulates a contractile cell behavior and cell density in a close unicellular relative of animals.
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In Capsaspora, coWts loss releases the Yorkie homolog to the nucleus more strongly than coHpo loss.
"Interestingly, we found that coWts-/- cells were significantly more likely to show nuclear mScarlet-coYki localization than coHpo-/- cells (Figure 1D)"
Assembling a Hippo: the evolutionary emergence of an animal developmental signaling pathway.
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The Monosiga brevicollis Hippo gene is predicted to be truncated.
"Further, the M. brevicollis Hippo gene contains an insertion that is predicted to truncate the protein at the beginning of the kinase domain [65]."