LAC1 (YKL008C; DGT1) notes

UniProt P28496, ceramide synthase subunit (TLC/Lag1 family, Lag1 paralog), with Lag1 and Lip1 forms the acyl-CoA ceramide synthase of the ER; selective for C26:0-CoA [UniProt:P28496].

• ER localisation by IF; with LAG1 needed for ER-to-Golgi transport of GPI proteins PMID:10198056.
• lag1 lac1 microsomes lack C26-CoA ceramide synthase PMID:11387200; PMID:11694577.
• Purified Lac1/Lag1 co-purify with activity; Lip1 is third subunit PMID:15692566.
• GPI-anchor ceramide remodelling also depends on Lag1/Lac1 PMID:11387200.

Pathway context

• YeastPathways PHS + VLC acyl-CoA -> phytoceramide (EC 2.3.1.24) correct; Lac1 is redundant with Lag1.