APELA notes

Biology

• ELABELA/Toddler is a secreted 32-aa peptide hormone and APLNR agonist.
• Zebrafish: heart and endoderm PMID:24316148; gastrulation motogen PMID:24407481.
• Human APJ signalling (cAMP, ERK, internalization, HUVEC angiogenesis) PMID:25639753.
• Human heart binding, an endogenous agonist PMID:28137936.
• Kd 0.51 nM and Gi signalling; rat fluid homeostasis PMID:25995451.
• Rat heart inotropy PMID:26611206.
• Mouse knockout cardiovascular defects PMID:28854362.
• Placental ELA and preeclampsia PMID:28663440.
• Cryo-EM of APJ-G protein with ELA bound PMID:35817871.

GOA calls

• Core, ACCEPT: hormone activity, apelin receptor binding, apelin receptor signaling pathway, extracellular region, heart development.
• GPCR signaling (IDA, cryo-EM) → MODIFY to apelin receptor signaling pathway.
• Adult heart development (ISS) → REMOVE. The GOA WITH/FROM is Q9WV08, mouse Aplnr: the receptor, not an APELA homolog.
• Non-core:
• Zebrafish gastrulation and endoderm ISS rows.
• Mouse vascular ISS rows.
• Placenta vessels.
• Trophoblast migration (IMP); angiogenesis (IMP); GPCR internalization (IMP).
• Rat heart contraction and ERK (ISS).
• No IBA rows; no NEW rows.
• Review round (PR #4156):
• Coronary vasculature now cites PMID:28890073 (Ela/Apj coronary migration defect).
• GO:1903589 (sprouting-angiogenesis EC proliferation) → MARK_AS_OVER_ANNOTATED. The donor paper reports a migration defect, and "proliferation" appears only twice in its text, both in background statements.
• Each mouse ISS row is adjudicated on its own donor evidence (found via QuickGO on P0DMC4).
• All 17 ISS rows carry propagation_review. The Q9WV08 row is PROPAGATION_BAD: the receptor's own adult heart IMP comes from PMID:28663440.